摘要
目的探讨RhoGDI2与EMT相关蛋白(E-cadherin、Vimentin)在结直肠癌组织中表达的临床意义以及它们之间的相关性。方法收集行手术切除且随访满5年的结直肠癌患者组织标本80例。应用免疫组化法检测标本中RhoGDI2与EMT相关蛋白的表达情况,分析它们与各临床病理参数间的关系,探讨RhoGDI2与EMT相关蛋白间的相关性。结果RhoGDI2、E-cadherin、Vimentin在结直肠癌组织中的阳性表达率分别为27.5%、23.6%、48.7%。结直肠癌组织中,RhoGDI2的表达与肿瘤的浸润深度、淋巴结转移、远处转移、TNM分期及5年生存率相关(P<0.05);E-cadherin的表达与肿瘤大小、分化程度、浸润深度、淋巴结转移、TNM分期及5年生存率相关(P<0.05);Vimentin的表达与肿瘤大小、分化程度、淋巴结转移、远处转移、TNM分期及5年生存率相关(P<0.05)。结直肠癌组织中,RhoGDI2与Vimentin的表达呈正相关(r=0.295,P<0.05);RhoGDI2、Vimentin与E-cadherin的表达呈负相关(r=-0.278、r=-0.309,P<0.05)。结论 RhoGDI2与EMT相关蛋白在结直肠癌组织中的表达密切相关,我们推测RhoGDI2可能通过促进EMT的发生导致了结直肠癌的侵袭、转移及不良预后。
Objective To investigate the clinical significance and correlation of RhoGDI2 and EMT-related proteins （E-cadherin,Vimentin）expression in colorectal carcinoma tissues.Methods The tissue specimens from 80 patients that were surgical resected for CRC and follow-up for over 5 years.The expression of RhoGDI2 and EMT-related proteins （E-cadherin,Vimentin）were assessed by immunohistochemistry.The associations of their expression with clinicopatho-logical parameters and themselves were analyzed.Results The positive expression rate of RhoGDI2,E-cadherin and Vi-mentin was 27.5%,23.6%,48.7% respectively in CRC tissues.In CRC tissues,the expression of RhoGDI2 were asso-ciated with the depth of invasion,lymph node metastasis,distant metastasis,TNM stage and 5-year survival（P 〈0.05）;the expression of E-cadherin were associated with the tumor size,differentiation,depth of invasion,lymph node metasta-sis,TNM stage and 5-year survival（P 〈0.05）;the expression of Vimentin were associated with the tumor size,differen-tiation,lymph node metastasis,distant metastasis,TNM stage and 5-year survival（P 〈0.05）.The expression of RhoG-DI2 with Vimentin showed positive correlation（r=0.295,P 〈0.05）;the expression of RhoGDI2,Vimentin with E-cad-herin showed negative correlation（r=-0.278,r=-0.309,P 〈0.05）.Conclusion The expression of RhoGDI2 was significantly associated with EMT-related proteins（E-cadherin,Vimentin）in CRC tissues.We speculate that RhoGDI2 may cause CRC invasion,metastasis and poor prognosis by promoting EMT occurrence.
出处
《中国实验诊断学》
2015年第9期1465-1469,共5页
Chinese Journal of Laboratory Diagnosis
基金
上海市卫计委面上项目(20114194)
吉林省发改委项目(3J113Z583429)
上海市长宁区卫计委面上项目(20114Y02003)
