摘要
目的:对三峡库区不同产地、不同品种栽培的重楼药材从化学成分角度进行品质评价,为筛选适生品种提供理论依据。方法:应用高效液相色谱法对重楼药材中重楼皂苷指标成分含量进行分析,通过紫外-可见分光光度法测定重楼药材中总皂苷、总黄酮和总多糖的含量,比较不同品种重楼药材的化学品质。结果:不同产地、不同品种的重楼属药材中含有重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅵ和重楼皂苷Ⅶ等甾体皂苷类成分的种类及含量各不相同,结合总皂苷、总黄酮和总多糖含量的测定结果,表明不同栽培品种重楼药材化学品质差异较大。结论:云南重楼和七叶一枝花在三峡库区规模化栽培是可行的,需加强其人工栽培技术的深入研究,同时非药典品种巴山重楼和长药隔重楼值得推广种植。
Objective： In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments,the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions. Methods： The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins,the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations. Results： Types and the contents of saponin Ⅰ,saponin Ⅱ,saponin Ⅵ and saponin Ⅶ were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins,the total content of flavonoids and the total content of polysaccharides,it was suggested that the chemical compositions existed obvious differences in different cultivars. Conclusion： It is proved that Paris polyphylla var. yunnanensis and Paris polyphylla var. chinensis could be cultivated on a large scale,but the techniques in artificial cultivation should be studied further. At the same time,Paris bashanensis and Paris polyphylla var. pseudothibetica are good cultivars for planting.
出处
《中药材》
CAS
CSCD
北大核心
2015年第5期894-898,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81260622)
重庆市教委科学技术研究项目(KJ131109)
万州区科委自然科学基金(201301024)
关键词
重楼
重楼皂苷
总皂苷
总黄酮
总多糖
质量评价
Paris
Saponins
Total saponins
Total flavonoids
Total polysaccharides
Quality evaluation
