摘要
从患病锦鲤体内分离得到1株细菌ML1,经人工感染后发现ML1具有较高的致病性,临床症状与自然发病症状一致,96 h的LD50为5.0×107cfu/m L。ML1菌株革兰染色呈阴性,杆状。16S rRNA基因序列分析结果显示,序列片段长度为1 419 bp,ML1菌株在系统发育树上与嗜水气单胞菌聚类,相似性达到100%。综合VITEK 2 Compact全自动微生物鉴定仪的生理生化特征分析、形态学观察、人工感染以及16S rRNA基因序列分析,将ML1菌株鉴定为嗜水气单胞菌。ML1菌株的最适生长p H为7~9,最适生长温度为28℃。在28℃200 r/min条件下的生长周期是：0~4 h为生长延迟期,4~10 h为对数生长期,10~24 h为稳定期,24~48 h为衰亡期。药敏试验表明,ML1菌株对多黏霉素B、环丙沙星、诺氟沙星等高度敏感,对磺胺异恶唑中度敏感,对麦迪霉素、氨苄西林、林可霉素等不敏感。研究结果为指导临床合理用药提供了科学依据。
One bacterial strain named ML1 was isolated from diseased carp. The artificial infection assay showed ML1 had a high virulence, and clinical symptoms of the artificially infected fish were similar to those in naturally infected fish. The LDsowas 5.0×10^7CFU/mL. The 16S rRNA gene sequence analysis showed that ML1 has a similarity of 100% with Aeromonas hydrophila. The best growth pH and temperature of strain ML1 were between 7 and 9 and 28 ℃, respectively. Its growth carve under the condition of 28℃ and 200 r/min was as fol- lows： the lag phase was 0 h -4 h, the log phase was 4 h - 10 h, the stationary phase was 10 h -24 h, and the decline phase was 24 h -48 h. The antibiotic sensitivity test showed that the strain ML1 had hypersensitivity to polymixin B, hydrochloric and norfloxacin; medium sensitivity to sulfafurazole ; nonsensitivity to midecamycin, ampicillin and cillimycin. This study is of guiding significance for the use of drugs in aquaculture.
出处
《畜牧与兽医》
北大核心
2015年第8期13-18,共6页
Animal Husbandry & Veterinary Medicine
基金
天津科技计划项目(14ZCDGNC00029)
天津科技计划项目(14ZCZDNC00010)
关键词
锦鲤
嗜水气单胞菌
生物学特性
鉴定
Cyprinus carpio haematopterus
Aeromonas hydrophila
biological characterization
identification
