摘要
目的:研究不同浓度配比(0%、1%、5%、10%)万古霉素/磷酸钙复合材料对大鼠骨髓间充质干细胞的毒性作用。方法:原代培养大鼠间充质干细胞并鉴定;采用CCK-8法测定不同浓度配比万古霉素/磷酸钙复合材料对大鼠骨髓间充质干细胞增殖的影响、TUNEL法测定细胞凋亡率、扫描电镜观察细胞形态学改变。结果:CCK-8结果显示,5%及10%万古霉素/磷酸钙复合材料显著抑制大鼠骨髓间充质干细胞增殖(P<0.05);TUNEL结果显示,5%及10%万古霉素/磷酸钙复合材料组细胞凋亡率显著增高(P<0.05);扫描电镜结果显示,高浓度万古霉素毒性作用下细胞失活,形态学发生显著变化;1%万古霉素/磷酸钙复合材料组对细胞影响相对于空白对照组无显著差异。结论:低浓度(1%)万古霉素/磷酸钙复合材料基本无细胞毒性,细胞相容性好。
Objective： To investigate the cytotoxicity ofvancomycin （Van）/calcium phosphate cement （CPC） composite materials on rat bone marrow mesenchymal stem cells （BMSCs）. Methods： Primary BMSCs were isolated, cultured and identified. The ceils were incubated with different concentrations （containing 0%, 1%, 5%, 10% Van） of Van/CPC composite materials. The cell viability of BMSCs was measured by CCK-8 method, and the cell apoptosis was detected by TUNEL method and the cell morphology was observed-by scanning electron microscope （SEM）. Results： 5% and 10% Van/CPC composite materials could significantly inhibit the proliferation ofBMSCs （P〈0.05）, and increase apoptosis （P〈0.05） and lead to higher cytotoxicity.1% Van/CPC exerted no obvious effect on the BMSCs compared to the blank control. Conclusion： Low concentration of Van/CPC had no cytotoxicity on rat and BMSCs performed good biocompatibility.
出处
《现代生物医学进展》
CAS
2015年第20期3813-3816,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(30901532
81371982)
关键词
万古霉素
磷酸钙
细胞毒性
感染
Vancomycin
Calcium phosphate cement
Cytotoxicity
Infection
