摘要
探究三七总皂苷制剂诱发类过敏反应的病理特点以及致类过敏反应的物质基础。采用小鼠类过敏试验模型,适应性饲养1 d后,将雄性ICR小鼠随机分成5个实验组,分别为空白组、阳性对照组、三七总皂苷制剂低、中、高剂量组,每组10只,一次性静脉注射含0.4%伊文思蓝(EB)的相应受试物,给药30 min后,记录各组动物耳廓蓝染数,进行耳廓蓝染程度评分,并定量测定耳廓EB渗出量。另做2批动物,只给予相应的受试物,30 min后,取外周血和肺脏,用ELISA法检测血清中相关的细胞因子,肺脏HE染色。结果表明,三七总皂苷制剂中、高剂量静脉注射均可引起小鼠耳廓血管通透性增高,耳廓明显蓝染,提示可能有致类过敏的作用。病理结果显示三七总皂苷制剂的类过敏反应与炎症反应密切相关,肥大细胞脱颗粒现象明显。组胺、VEGF以及TNF-α的含量在三七总皂苷制剂中、高剂量时明显升高(P<0.05,P<0.01)且呈现出剂量依赖关系,与耳廓蓝染程度保持一致,而IL-6和IL-10含量在三七总皂苷制剂低、中剂量时没有明显升高,当其增为临床4倍浓度8倍剂量时较空白组均明显升高(P<0.05,P<0.01)。其产生类过敏作用可能与组胺,VEGF,TNF-α有关,但可能当其类过敏程度达到一定高的程度时,IL-6和IL-10才可能在类过敏反应中发挥作用。
The experiment is designed to explore pathological festures and material basis of pseadoanaphylactoid reaction induced by notoginseng total saponin preparation. Mouse pseadoanaphylactoid reaction was used, 50 ICR mice were randomly assigned to control group, positive medicine group, notoginseng total saponin preparation low-dose group, notoginseng total saponin preparation middledose group, notoginseng total saponin preparation high-dose group on average. They are treated by intravenous injection of test substance solutions containing 0.4% Evans blue （EB）. 30 rain later, scores of ear blue staining and quantitation of ear EB exudation were recorded. Another two experiment were repeated in the same way excluding EB, just to detect the related cytokines in serum using ELISA. We found that the scores of pseudoanaphylactoid reaction in notoginseng total saponin preparation injection middle-dose group and high-close group was evidently higher than that in control group, suggesting that notoginseng total saponin preparation injection may be can lead to pseadoanaphylactoid reaction. HE staining showed that pseadoanaphylactoid reaction induced by notoginsengtotal saponin preparation injection is related to inflammation. Histamine, VEGF and TNF-α levels in notoginseng total saponin preparation mid- dle-dose group and high-dose group significantly increased （P 〈 0. 05, P 〈 0.01 ）than control group and showed a dose-dependent manner as well as consistent with the degree of ear blue dye. While IL-6 and IL-10 content did not increase significantly in notoginseng total saponin preparation low-dose group and middle-dose group, but they significantly higher than control gronp（P 〈0. 05, P 〈0. 01 ）when it increased to quadrupe clinical concentrations, eight times of the clinical dose. So pseadoanaphylactoid reaction caused by notoginseng total saponin preparation may be related to histamine, VEGF, TNF-α, and it is possible that IL-6 and IL-10 can play a role when pseadoanaphylactoid reaction achieve a certain high degree.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第14期2732-2736,共5页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2012ZX09101201)
关键词
类过敏反应
三七总皂苷制剂
物质基础
血管通透性
pseadoanaphylactoid reactions
notoginseng total saponin preparation
material basis
vascular permeability
