摘要
微生物对外界环境的变化具有灵活的适应性,通过代谢网络的通量可变性分析可以对代谢灵活性进行量化,得到影响菌体代谢灵活性的因素。使用代谢流分析软件COBRA Toolbox,对克雷伯氏杆菌基因组尺度代谢网络进行通量平衡分析与通量可变性分析,分别得到内部反应的可变性、交换反应的可变性和生长速率的可变性等3个因素对菌体代谢灵活性的影响程度,其中生长速率的可变性是影响代谢灵活性的主要因素,调控菌体至次优模式下生长可使其具有更大的代谢灵活性。通过进一步研究生长速率对通量可变性的影响,找到提高代谢灵活性的两个必要条件,从而增强菌体应对外界环境改变的能力。
Microorganisms can make flexible adaptability in response to the external changing environment. Metabolic flexibility can be quantified through flux variability analysis so as to find the factors affecting meta- bolic flexibility. In this work, flux balance analysis and flux variability analysis against genome-scale metabolic network were used to calculate three aspects of factors affecting metabolic flexibility of Klebsiella pneurnoniae such as internal reaction variability, exchange reaction variability, growth rate variability with COBRA Tool- box. Results showed that growth rate variability was the major factor affecting metabolic flexibility and control- ling growth rate to suboptimal could make greater metabolic flexibility. Effect of growth rate variability on flux variability was further studied, and summed up two necessary conditions for improving metabolic flexibility and enhancing the adaptability for external environment.
出处
《化学与生物工程》
CAS
2015年第8期43-47,共5页
Chemistry & Bioengineering
基金
山东省自然科学基金资助项目(ZR2013BM001)
