摘要
目的观察参茸养心益肾胶囊对血管性痴呆(AD)患者脑脊液和外周血p38MAPK通路主要因子P-p38MAPK/p38MAPK蛋白表达的影响。方法血管性痴呆患者31例为病例组,给予参茸养心益肾胶囊治疗,对比治疗前后简易智能量表(MMSE)评分和长谷川痴呆量表(HDS)评分变化;治疗前后以Western blot检测患者脑脊液和空腹静脉血P-p38MAPK/p38MAPK蛋白表达量。以同期入院的26例非痴呆患者为对照组,进行对比分析。结果病例组治疗前MMSE、HDS评分分别为(21.3±3.5)分、(19.6±3.4)分,治疗后分别上升至(28.2±4.8)分、(24.3±4.2)分,差异有统计学意义(P<0.01)。治疗前病例组脑脊液、血P-p38MAPK/p38MAPK表达水平为(0.983±0.106)、(0.867±0.113),明显高于对照组的(0.248±0.042)及(0.336±0.146)(P<0.01);治疗后病例组P-p38MAPK蛋白表达量明显降低,P-p38MAPK/p38MAPK表达水平显著低于治疗前(P<0.05)。结论参茸养心益肾胶囊可能通过抑制血管性痴呆患者p38MAPK磷酸化激活,干预p38MAPK信号通路级联反应而获得临床疗效。
Objective To investigate the effect of ginseng yangxin yishen capsule on P-p38MAPK/p38MAPK protein expression in cerebrospinal fluid and peripheral blood from patients with vascular dementia. Methods Thirty-one patients with vascular dementia were recruited as case group and were giver ginseng yangxin yishen capsules. Mino-mental State Examination（MMSE） and Hasegawa Dementia（HDS） scores were evaluated and compared before and after treatment. P-p38MAPK/p38MAPK protein expression in cerebrospinal fluid and peripheral blood were detected by Western blot. Twenty-six non-vascular dementia patients over the same period served as control. Results In case group the MMSE and HDS scores increased to 28.2±4.82 and 24.3±4.19 from 21.3±3.54 and 19.6±3.44, respectively, both with significant difference before and after treatment （P〈0.05）. Before treatment, P-p38MAPK/p38MAPK protein expressions in cerebrospinal fluid and peripheral blood in case group were higher than those in control group （cerebrospinal： 0.983±0.106 vs 0.248±0.042,P〈0.05; peripheral blood： 0.867±0.113 vs 0.336±0.146, P〈0.05）; after treatment, the expression of P-p38MAPK protein arid P-p38MAPFk/p38MAPK level in case group significantly decreased compared witll those befoRe treatment （P〈0.05）. Conclusion Ginseng yangxin yisheng capsule may treat vascular dementia by interfering p38MAPK signal pathway via inhibiting activation of P- p38MAPK.
出处
《浙江中西医结合杂志》
2015年第8期727-729,732,共4页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
