摘要
目的探讨淋巴细胞各亚群及细胞免疫应答产物干扰素-γ(IFN-γ)水平在弓形虫感染患儿细胞免疫应答中的变化趋势及临床意义。方法选取2011年1月至2014年4月检测出Tox-Ig M阳性的34例患儿为Tox-Ig M阳性组,同时选取既往感染的TOX-Ig G阳性患儿12例为TOX-Ig G阳性组,另选取行健康体检的健康儿童54例为对照组。应用流式细胞仪检测各组外周血淋巴细胞CD4+、CD8+、CD19+和NK细胞水平,并计算CD4+/CD8+比值;采用酶联免疫吸附试验(ELISA)检测各组患儿血清中IFN-γ的水平。结果 Tox-Ig M阳性组患儿CD4+、CD4+/CD8+比值明显低于TOX-Ig G阳性组和对照组,CD8+、NK细胞和血清IFN-γ水平高于TOXIg G阳性组和对照组(P<0.05)。TOX-Ig G阳性组患儿血清IFN-γ水平高于对照组(P<0.05)。Tox-Ig M阳性组患儿CD8+水平与血清IFN-γ水平呈正相关(r=0.756,P<0.05)。结论 CD4+、CD8+以及NK细胞等免疫细胞及其免疫应答产物IFN-γ水平变化在弓形虫感染过程中发挥着重要的作用。
ObjectiveTo study the changes and significance of lymphocyte sunsets and serum interferon-γ（IFN-γ） levels in children with toxoplasma infection.MethodsThirty-four children who were newly diagnosed with toxoplasma infection （TOX-IgM＋ group） between January 2011 and April 2014, 12 children who had ever been diagnosed with toxoplasma infection （TOX-IgG＋ group）, and 54 healthy children （control group） were enrolled. The percentages of CD4＋, CD8＋, CD19＋ and NK cells in peripheral blood lymphocytes were detected by lfow cytometry. Serum levels of IFN-γ were measured using ELISA.ResultsThe percentages of CD4＋ cells and the CD4＋/CD8＋ ratio in the TOX-IgM＋ group were signiifcantly lower than in the TOX-IgG＋ and control groups, while the percentages of CD8＋ and NK cells and serum IFN-γ levels were signiifcantly higher than in the other two groups （P〈0.05）. The TOX-IgG＋ group had higher serum IFN-γ levels than the control group （P〈0.05）. There was a positive correlation between the percentage of CD8＋ cells and serum IFN-γ levels in the TOX-IgM＋ group （r=0.756;P〈0.05）.ConclusionsCD4＋, CD8＋ and NK cells may play important roles in the resistance against toxoplasma infection by promoting the secretion of cytokines.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2015年第7期687-691,共5页
Chinese Journal of Contemporary Pediatrics
关键词
淋巴细胞亚群
Γ干扰素
弓形虫
儿童
Lymphocyte subsets
Interferon-γ
Toxoplasma gondii
Child
