摘要
目的 研究利用隐形纳米脂质体包裹的Mesothelin抗体修饰的超小超顺磁性氧化铁（MSLN-USPIO）作MRI靶向对比剂诊断裸鼠皮下种植胰腺癌的可能性和价值.方法 制作多时间节点荷人胰腺癌裸鼠模型,分别使用USPIO和MSLN-USPIO作为MRI对比剂进行3.0T MRI扫描,比较二者对最小种植瘤的显示阳性率、种植瘤组织T2WI的负性强化率;通过种植瘤标本普鲁士蓝染色,观察种植瘤组织中铁离子沉积.结果 两种对比剂显示最小肿瘤的阳性率差异无统计学意义;使用USPIO增强后左、右腋窝肿瘤的负性强化率分别为（12.29±7.45）％和（11.06±5.91）％,使用MSLN-USPIO增强后左、右腋窝肿瘤的负性强化率分别为（33.88±6.09）％和（43.29±11.64）％,两组种植瘤增强前后的信号值差异及同一部位种植瘤增强后的负性强化率差异均有统计学意义（P值均＜0.05）,且MSLN-USPIO组的负性强化率显著高于USPIO组（P＜0.05）.普鲁士蓝染色显示MSLN-USPIO组种植瘤组织内的铁颗粒沉积明显多于USPIO组.结论 MSLN-USPIO的增强效果明显优于USPIO,可作为荷瘤裸小鼠胰腺癌MRI靶向对比剂。
Objective To study the potential and value of ultrasmall superparamagnetic iron oxide （USPIO） conjugated by mesothelin antibody as MRI targeting contrast agent for diagnosis of implanted human pancreatic carcinomas in nude mouse.Methods Nude mouse tumor models bearing multiple human pancreatic carcinomas at different time points was established and they were randomized into two groups,and USPIO or MSLN-USPIO were used as contrast enhanced agents in the 3.0T MRI scan,respectively,then the positive detection rates for smallest tumors,and the signal intensity of tumors in T2 mapping images of both unenhanced and contrast enhanced scanning and the negative enhancement rate were measured,then Prussian blue staining was performed in alI the tumor specimens to observe the difference of Fe3 ＋ ion deposition.Results There was no statistical significance between USPIO group and MSLN-USPIO group in the positive detection rates for smallest tumors.In USPIO group,the negative enhancement rate of left or right axilla tumors was （12.29 ±7.45）% and （11.06 ±5.91）%,and they were （33.88 ±6.09）% and （43.29 ± 11.64）% in MSLN-USPIO group.There was statistical significance in the difference of signal intensity between unenhanced and contrast enhanced in left or right axilla tumors （P 〈 0.05）,and the negative enhancement rate in MSLN-USPIO group was significantly higher than that in USPIO group （P 〈0.05）.The Fe^3＋ ion deposition in tumors＇ tissue in MSLN-USPIO group was significantly more than that in USPIO group.Conclusions The enhanced effect of MSLN-USPIO is superior to USOPIO,and it can be a tumor targeted MR contrast enhanced agent for the diagnosis of pancreatic carcinoma in nude mouse.
出处
《中华胰腺病杂志》
CAS
2015年第3期177-181,共5页
Chinese Journal of Pancreatology
基金
国家自然科学基金资助项目（30970801）
关键词
胰腺肿瘤
超微超顺磁性氧化铁
小鼠
裸
磁共振成像
Pancreatic neoplasm
Ultrasmall superparamagnetic iron oxide
Mice,nude
Magnetic resonance imaging
