摘要
以油樟、大叶樟、香樟、天竺桂4种樟属植物叶水浸提液为诱导子,添加到油樟悬浮培养系中,研究诱导子对油樟悬浮培养细胞生长及挥发性物质积累的影响。结果表明,4种樟属植物叶水浸提液对油樟悬浮细胞生长无明显影响。不同樟属植物叶水浸提液对挥发性物质影响不同。对于总的挥发性物质的积累,有促进作用的是天竺桂与香樟叶浸提液,添加量分别是4%和1%时促进作用最强,其峰面积分别为空白对照组的1.78和1.45倍;对1,8-桉叶油素的积累,有促进作用的是天竺桂叶浸提液,添加量为1%时,促进作用最强,其峰面积为空白对照组的1.46倍;对于γ-松油烯的积累,4种叶浸提液均有促进作用,大叶樟、天竺桂和香樟水提液添加量1%时促进作用最强,其峰面积分别0.39、0.27和0.32 p A,油樟水提液添加量4%时促进作用最强,其峰面积为0.27 p A(对照中无);对于α-松油醇的积累,有促进作用的是天竺桂与香樟水提液,添加量为0.25%时促进作用最强,其峰面积分别为空白对照组的1.62和1.24倍。本研究在一定程度上揭示了油樟油成分积累相关促进因子情况,为进一步研究奠定基础。
Water extracts of 4 Cinnamomum plant leaves including Cinnamomum longepaniculatum N.Chao ex H.W.Li., Cinnamomum parthenoxylum, Cinnamomum camphora and Cinnamomum japonicum Sieb.were added to the cell suspension cultures of Cinnamomum longepaniculatum to study their effect on the cell growth and volatile metabolites accumulation.The results showed that the 4 targeted water extracts of Cinnamomum plant leaves had no effect on cell growth. However,leaf extracts had different effects on volatile metabolites accumulation in the cell suspension cultures of C.longepaniculatum. C.japonicum extract （4%） and C.camphora extract （1%） increased the total content of volatile metabolites in the cell suspension cultures of C.longepaniculatum by 78% and 45%, respectively.1% of C.japonicum extract was the optimal concentration which increased the accumulation of 1,8-cineole by 46%.Leaf extracts promoted the accumulation of γ-terpinene in the cell suspension cultures of C.longepaniculatum. 1% of C.parthenoxylum, C.camphora and C.japonicum extracts increased the peak area of γ-terpinene from 0 pA to 0.39,0.27 and 0.32,respectively.4% of C.longepaniculatum extract increased the peak area of γ-terpinene from 0 pA to 0.27 pA. 0.25% of C.japonicum and C.camphora extracts had the strongest promotion effects for α-terpineol.The content of α- terpineol was 1.62 and 1.24 times as blank. This study revealed that leaf extracts from Cinnamomum plant leaves affected the accumulation of volatile metabolites in the cell suspension cultures of C.longepaniculatum, which laid a foundation for further study.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第7期1146-1150,1198,共6页
Natural Product Research and Development
基金
四川省教育厅项目(08ZA002)
四川省科技计划项目(2010JY0093)
宜宾市科技局项目(2011ZSF008)
关键词
樟属
油樟
悬浮细胞培养
挥发性物质
1
8-桉叶油素
γ-松油烯
Α-松油醇
Cinnamomum plants
Cinnamomum longepaniculatum N. Chao ex H. W. Li.
suspension cell culture
volatileproducts
1,8 -cineole
γ-terpinene
α-terpineol
