摘要
目的从血管新生的角度,探讨益心解毒方对气虚血瘀证心衰大鼠心肌保护的作用机制。方法利用左冠状动脉结扎术制备大鼠心梗后心衰动物模型,通过宏观表征判定该疾病模型的证候属性,将成模后存活大鼠随机分为8组:假手术组,模型组,益心解毒方高、中、低剂量组,中西结合组,福辛普利组和芪苈强心组。治疗4周后取梗死边缘区组织进行Western Blot及RT-PCR法检测血小板—内皮细胞黏附分子(又称CD31)和血管内皮生长因子的表达。结果 Western Blot结果显示:模型组大鼠心肌组织中CD31蛋白表达量较假手术组显著下降(P=0.004),而高剂量组(P=0.042)、低剂量组(P=0.027)和芪苈强心组(P=0.015)与模型组相比均可提高心肌组织中CD31的表达。PCR结果显示:与假手术组相比,大鼠心肌组织的血管内皮生长因子表达在模型组(P=0.047),高剂量(P=0.023),中西结合组(P=0.008)均显著升高;而低剂量组大鼠心肌组织中血管内皮生长因子的表达显著低于模型组(P=0.004)和其它治疗组(P<0.05)。结论益心解毒方对心梗所致气虚血瘀证心衰大鼠的心肌保护作用,可能是通过促进血管新生实现的,其机制可能与血管内皮生长因子有关,且具有剂量相关性。
Objective To clarify myocardial protection mechanism of Yixin Jiedu Formula（ YXJDF） on chronic heart failure rats with qi deficiency and blood stasis syndrome based on angiogenesis.Methods Rat models of heart failure were established by using left coronary artery ligation. The syndrome of rats was evaluated by macroscopic symptoms. All the survival rats after model establishment were randomly divided into 8 groups： sham-operated group,model group,high,middle and low dose of YXJDF groups,combination therapy group,fosinopril group and Qili Qiangxin（ QLQX） group. 4 weeks after treatment,western blot and RT-PCR were used to test the expression of CD31 and vascular endothelial growth factor（ VEGF） in infarction border zone. Results The results of western blot showed that the expression of CD31 in cardiac muscle tissue of rats in model group was significantly decreased comparedwith the sham operated group（ P = 0. 004）,while the protein expression of CD31 in myocardial tissue of rats in the high and low dosage of Yixin Jiedu formula group（ P = 0. 042,P = 0. 027） and Qili Qiangxin capsule group（ P = 0. 015） was increased significantly. The RT-PCR results indicated that,compared with the sham operated group,the expression of VEGF in myocardial tissue of rats in model group（ P =0. 047）,high dose of Yiqi Jiedu formula group（ P = 0. 023） and combination therapy group（ P = 0.008） were dramatically increased,while the expression of VEGF in myocardial tissue of rats in low dose of Yiqi Jiedu formula group was lower than that of rats in model group（ P = 0. 004） and the other treatment groups（ P〈0. 05）. Conclusion Yixin Jiedu formula could protect the cardiac muscle of rats with qi deficiency and blood stasis syndrome induced by myocardial infarction through promoting angiogenesis possibly,and the mechanism might be relate to VEGF family,and the protective effect changes with different doses.
出处
《环球中医药》
CAS
2015年第4期419-423,共5页
Global Traditional Chinese Medicine
基金
国家科技重大专项"重大新药创制"专项(2012ZX09103201-011)
国家"十二五"科技支撑计划(2012BA129B07)
国家自然科学基金(84170191)
关键词
益心解毒方
慢性心力衰竭
气虚血瘀证
血管新生
Yixin Jiedu Formula
Chronic heart failure
Qi deficiency and blood stasis syn-drome
Angiogenesis
