摘要
作为一种新颖高效的光谱技术,傅里叶变换红外光谱成像(Fourier transform infrared spectroscopic imaging,FTIRI)可以在高分辨率下获得样品的组成和结构的信息,已开始深入应用到生物医学光子学研究领域。本文采用FTIRI技术进行关节软骨的主成分(胶原蛋白和蛋白多糖PG)的红外光谱成像及分析方法的研究。首先对多组软骨切片进行FTIR成像及红外光谱分析,进而采用特征峰强度积分法和二次求导方法分别对这些特征带(Amide I,II,III and Sugar)进行表征,给出两种主成分或特征基团含量随软骨深度的变化关系。其次采用主成分回归方法定量计算二主成分的浓度,将这些浓度和对应位置的光谱特征峰积分面积及二次求导后的峰面积进行相关性研究。结果显示Amide I和Amide II特征峰的积分强度和胶原蛋白浓度的相关性最高。二次求导后Sugar带强度与蛋白多糖浓度的相关性最高。这些结果说明Amide I和Amide II带的积分强度适合用于定性表达胶原蛋白的含量及其随深度的变化,而Sugar带的二次求导强度更适于定性表达蛋白多糖的含量及其随深度的变化。Amide III带积分和二次求导既不适合表征胶原蛋白也不适合表征PG的含量。这项研究为关节软骨和骨关节炎早期研究提供了更加快捷实用的方法,从而有利于早期骨关节炎的判断和修复监测。
As a novel and effective spectroscopic analytical tool,Fourier transform infrared spectroscopic imaging（FTIRI）can obtain composition and structure information of samples at high resolution and has been applied in biomedical photonics field.FTIRI is employed to analyze the characteristic IR bands of principal components of five articular cartilages and their attribution by integrated and second derivative methods.The depth dependent profiles of integrated absorbance and second derivative spectral intensity of these IR bands are lineally fitted to those of the concentrations of collagen and proteoglycan that are predicted by principal component regression algorithms.The correlation coefficients by linear fit suggest that the correlation coefficient（R）between the integrated absorbance of amideⅠ（amideⅡ）and collagen concentration（RA-amideⅠ RA-amideⅡ）is much higher than that between amideⅢ absorbance and collagen concentration（RA-amideⅢ）,namely,RA-amideⅠ,RA-amideⅡ〉RA-amideⅢ,as well as the correlation coefficient between the second derivative spectral intensity of sugar band and PG concentration better than that between integrated absorbance of sugar band and PG concentration,RD-sugar〉RA-sugar.It is concludedthat the integrated intensities of amide I and amide II are very suitable to qualitatively represent the content and depth dependence of collagen.The second derivative spectral intensities of sugar band is much fit to represent the contents and depth dependences of PG,respectively.The integrated or the second derivative spectral intensity of amideⅢ band is unfit to represent either collagen or PG content in cartilage.This study provides a simpler and quicker method for the investigation on cartilage and early-stage osteoarthritis,which will be helpful for the diagnosis and recovery monitoring of early-stage osteoarthritis.
出处
《南京航空航天大学学报》
EI
CAS
CSCD
北大核心
2015年第3期421-427,共7页
Journal of Nanjing University of Aeronautics & Astronautics
基金
国家自然科学基金(61378087)资助项目
高等学校博士学科点专项科研基金(20133218120017)资助项目
南京航空航天大学2014~2015年本科专业建设(1403ZJ04XX03)资助项目
关键词
红外光谱特征峰
二次求导
关节软骨
胶原蛋白
蛋白多糖
infrared characteristic band
second derivative
articular cartilage
collagen
proteoglycan
