摘要
建立一种快速测定龙血竭含片中龙血素A、B两种有效成分的高效液相色谱（HPLC）方法。样品经甲醇超声提取,自制固相小柱净化富集,采用kromasil C18色谱柱（200×4.6mm,5μm）进行分离,以乙腈-0.1%乙酸溶液（37∶63,V/V）为流动相进行洗脱,检测波长为280nm。考察了不同流速、柱温和不同规格色谱柱对分离效果的影响,优化了实验条件。优化条件下,龙血竭含片中主要成分龙血素A、B均达到基线分离,龙血素A在0.1030~1.5450μg、龙血素B在0.0426~0.6390μg范围呈良好线性关系,相关系数r2均为1.0000,回收率为97.81%~101.77%,相对标准偏差（RSD）为0.3%~0.5%。该方法快速、准确,可满足实际检测需要。
A high performance liquid chromatography（HPLC）method was developed for the rapid determination of loureirin A and B in dragon＇s blood lozenges.The samples were extracted with methanol by the ultrasonic extraction method,then purified and enriched through homemade solid-phase column.The enrichments were separated on a kromasil C18（200×4.6 mm,5μm）chromatographic column by using acetonitrile-0.1% acetic acid（37∶63,V/V）as mobile phase with the UV detection wavelength at 280 nm.The effects of experimental conditions,such as flow rate,column temperature,and different type of chromatographic column on separation efficiency were investigated.Under the optimal conditions,the main ingredients of loureirin A and B in dragon＇s blood lozenges reached the baseline separation.The method showed good linearity with correlative coefficient of 1.0000.The recoveries were 97.81%-101.77%,and the relative standard deviations were 0.3%-0.5%.This method was rapid and accurate which can meet the needs of real sample testing.It provided the basis for the quality control research of dragon＇s blood lozenges.
出处
《分析科学学报》
CAS
CSCD
北大核心
2015年第3期363-366,共4页
Journal of Analytical Science
基金
国家自然科学基金(No.31260399)
云南省创新人才基金(No.2011C078)
昆明理工大学分析测试基金(No.20140738)
关键词
龙血竭含片
高效液相色谱
龙血素A
龙血素B
Dragon＇s blood lozenges
High performance liquid chromatography
Loureirin A
Loureirin B
