摘要
目的 采用益生菌制剂对胆汁淤积性肝病患儿进行治疗,以了解其对肠道菌群及相关细胞因子的影响.方法 1.将2010年10月至2011年6月在广西医科大学第一附属医院住院的84例胆汁淤积性肝病患儿采用简单数字表随机法分成益生菌干预组和非干预组.采用SYBR Green Ⅰ型实时荧光定量聚合酶链反应检测肠道细菌,比较益生菌干预组与非干预组肠道3种代表细菌数量的变化.2.检测2组治疗前后肝功能、血氨水平、胆固醇等指标;同时采用酶链免疫吸附试验测定血清转化生长因子-β1、肿瘤坏死因子-α（TNF-α）和白细胞介素-6 （IL-6）3种细胞因子水平.结果 1.益生菌干预组治疗前后比较,粪便中双歧杆菌、乳酸杆菌及双歧杆菌/大肠杆菌比值（B/E）均显著增加（P均＜0.01）,大肠杆菌数量减少（P＜0.05）;非干预组双歧杆菌、乳酸杆菌、大肠杆菌数量及B/E差异均无统计学意义（P均＞0.05）.2.2组治疗后总胆红素、结合胆红素、γ-谷氨酰转移酶、丙氨酸转移酶、总胆汁酸、血氨、碱性磷酸酶水平均有好转（P均＜0.05）;益生菌干预组治疗后TNF-α及IL-6水平下降（t=7.31,P =0.00;t-2.90,P=0.01）,非干预组中差异无统计学意义;且血氨水平在益生菌干预组下降更明显（t=-8.37,P=0.00）.3.B/E与IL-6水平呈负相关（r=-0.796,P=0.01）.结论 益生菌制剂干预治疗对胆汁淤积性肝病肠道菌群的恢复和免疫平衡具有一定的作用.
Objective To explore the effects of application of probiotics on intestinal flora and related cytokines in infants with cholestatic liver disease.Methods （1） Eighty-four infants with cholestatic liver disease had been hospitalized from October 2010 to June 2011 in the First Hospital of Guangxi Medical University.The infants with cholestatic liver disease were randomly divided into the probiotic intervention group and the non-probiotic intervention group.Quantification of intestinal bacteria was detected by SYBR Green Ⅰ real-time fluorescent quantitative polymerase chain reaction,then the number of 3 kinds of bacteria before and after the treatment was compared.（2） The indices of liver function,blood ammonia,cholesterol were detected.The levels of serum transforming growth factor-β1,tumor necrosis factor-α（TNF-α）and interleukin-6 （IL-6） were measured by enzyme linked immunosorbent assay.Results （1） The number of bifidobacterium,lactobacillus and bifidobacterium/escherichia coli （B/E） were significantly increased （all P 〈 0.01）,whereas the number of escherichia coli was significantly decreased in the probiotic intervention group（P 〈 0.05）,however,there were no differences in the non-probiotic intervention group（all P 〉 0.05）.（2）The indices of total billirubin,direct billirubin,γ-Glutamyltransferase,total bile acid,alanine aminotransferase,blood ammonia,alkaline phosphatase were significantly improved after therapy in 2 groups （all P 〈 0.05）.The levels of TNF-α and IL-6 were significantly decreased in the probiotic intervention group （t--7.31,P =0.00;t =-2.90,P =0.01）,but there were no differences in the non-probiotic intervention group.The level of BA was significantly decreased in the probiotic intervention group than the non probiotic intervention group （t =-8.37,P =0.00）.（3） The B/E value were significantly inverse correlated with level of serum IL-6 （r =-0.796,P =0.01）.Conclusions It may help to restore the intestinal flora and balance the immune function in infants with cholestatic liver disease after application of probiotics.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2015年第12期945-948,共4页
Chinese Journal of Applied Clinical Pediatrics
基金
广西卫生厅自筹经费科研项目（桂卫Z2011341）
