摘要
建立采用涡旋辅助浊点萃取滇重楼提取物中4个偏诺皂苷类成分，以非离子表面活性剂脂肪醇聚氧乙烯醚AEO-9为萃取剂，V（乙睛）：旷（水）=38：62为流动相，对富集的偏诺皂苷进行高效液相色谱快速分离的方法．最优实验条件为，AEO-9用量为110μL，正戊酸用量为150IxL，pH为9，平衡温度为40℃，平衡时间为15min．结果表明，检测出滇重楼中4个偏诺皂苷VI，VII，Pa及H的质量分数分别为0．9923％，1．3603％，3．3444％及1．6663％，检测限达3．2～4．0μg／L，RSD低于0．5％，定量限为6．6～8．3μg／L，线性范围为10～500μg／L，萃取的富集倍数达到23倍，萃取的回收率为98．68％～102．46％．
To establish a method using vortex-assisted cloud point extraction for four pennogenin Ingredients of Paris polyphylla Sm., taking nonionic surfactant primary alcobol ethoxylate (AEO -9)as the extractant,using a mixture of acetonitrile-water (38 ∶62,by volume)as the mobile phase,the enrichment Pennogenin Ingredients was rapid performed by high performance liquid chromatography (HPLC).Under the optimized conditions,the volume of AEO -9 was 110 μL,the volume of n-Valerate was 150 μL,pH was 9.0,the equilibration time and temperature were 40 ℃ and 15 min,respectively.The result shows that the contents of four Pennogenin Ingredients of Paris polyphylla Sm.VI,VII,Pa and H were 0.992 3%,1.360 3%,3.344 4% and 1.666 3%,respectively.The detection of limits (LOD)was 3.2-4.0 μg/L;the relative standard deviations (RSD)was below 0.5% (n =9 );the limit of quantification (LOQ)was 6.6-8.3 μg/L;the linear range was 10-500 μg/L;the enrichment factor was 23 and the relative recoveries was ranged from 98.68% to 102.46%.
出处
《昆明学院学报》
2015年第3期56-60,共5页
Journal of Kunming University
基金
昆明学院科学研究资助项目(XJL12015)
关键词
浊点萃取
AEO-9
滇重楼
偏诺皂苷
cloud point extraction
AEO-9
Paris polyphylla Sm.
Pennogenin
