摘要
目的建立甘草配方颗粒中甘草酸的含量测定方法。方法采用C18色谱柱,以甲醇-0.2mol·L-1醋酸铵（65∶35,醋酸调节p H值至4.0）为流动相,流速为1.0m L·min-1,检测波长为250nm。结果甘草酸在0.492∽7.872μg之间呈现良好的线性关系,r=0.9999;平均加样回收率为99.9%,RSD=1.2%（n=6）。结论本方法重复性好,能准确测定甘草配方颗粒中甘草酸的含量。
OBJECTIVE To establish the method for determining of glycyrrhizic acid in Glycyrrhizae Granula by HPLC.METHODS C18 column was used with the mobile phase consisted of methanol-0.2mol· L-1 ammonium acetate （65∶35,adjusted pH to 4.0 with CH3COOH）,the flow rate was 1.0mL· min -1,the detection wavelength was 250nm.RESULTS the calibration curve was linear at a range of 0.492∽7.872μg for glycyrrhizic acid （ r=0.9999）,the average recovery was 99.9%and RSD was 1.2%（n=6）.CONCLUSION the method was accurate with a good reproducibility and can be used as a quantitative analysis of Glycyrrhizic acid in Glycyrrhizae Granula.
出处
《海峡药学》
2015年第5期59-60,共2页
Strait Pharmaceutical Journal
