摘要
以笃斯越橘(Vaccinium uliginosum)成熟果实为试材,以钼蓝比色法测定笃斯越橘果实还原型抗坏血酸(Reduced ascorbic acid,As A)含量进行条件优化。结果表明,3%偏磷酸-乙酸用量为100μL、5%硫酸和5%钼酸铵用量均为200μL、以去离子水补充至1 500μL,30℃干热恒温浴显色40 min,取出室温下放置1 h后,在700 nm下测定,所得数据稳定,准确性适合用于笃斯越橘果实As A含量的测定;测得含量为(40.20±6.23)mg/100 g FW。
Ripe fruit of Vaccinium uliginosum were used to establish and optimize molybdenum blue colorimetric method of determining the content of reduction-type VC （AsA）. The results showed that the dosage of 3% metephosphoric acidacetic acid, 5% sulfuric acid, 5% molydate-ammonium was 100, 200, 200 μL, repectively in the 700 nm wavelength. Distilled water was filled to 1 500 μL. It was coloured with dry heat and constant temperature bathed at 30 ℃ for 40 rain,then taken out at room temperature and measured after 1 h. The data obtained were stable and accurate, suitable for determining the content of reduction-type VC（AsA）of Vaccinium uliginosum fruit, with the average content of （40.20±6.23）mg/100 g FW.
出处
《湖北农业科学》
2015年第7期1713-1716,共4页
Hubei Agricultural Sciences
基金
吉林省科技支撑计划重点项目(20120251)
