摘要
目的检测F框／WD-40域蛋白7（FBXW7）在食管鳞状细胞癌（简称食管鳞癌）组织中的表达及临床意义，初步探讨其与患者临床病理特征和预后的关系。方法采用免疫组织化学方法检测FBXW7蛋白在90例食管鳞癌和20例相应的癌旁正常组织及40例食管上皮内瘤变组织中的表达情况，采用Western blot法及半定量逆转录聚合酶链反应（RT-PCR）检测40例食管鳞癌组织和癌旁正常组织中FBXW7蛋白和mRNA表达水平，分析FBXW7表达与食管鳞癌患者临床病理特征及预后的关系。结果在食管鳞癌组织、癌旁正常组织、食管低级别上皮内瘤变组织和高级别上皮内瘤变组织中，FBXW7蛋白的阳性表达率分别为40．0％（36／90）、85．0％（17／20）、77．3％（17／22）和33．3％（6／18），差异有统计学意义（P〈0．001）。40例食管鳞癌组织和癌旁正常组织中，FBXW7蛋白表达水平分别为0．283±0．055和0．657±0．091，差异有统计学意义（P〈0．001）。40例食管鳞癌组织和癌旁正常组织中，FBXW7 mRNA相对表达水平分别为0．863±0．962和1．813±0．512，差异有统计学意义（P〈0．05）。单因素分析显示，FBXW7蛋白表达与肿瘤TNM分期、分化程度、浸润深度和淋巴结转移有关（均P〈0．05）。Cox多因素分析显示，仅淋巴结转移为食管鳞癌患者的独立预后因素（P〈0．05）。90例患者中，FBXW7蛋白阳性表达和阴性表达患者的5年生存率分别为67．6％和39．3％，差异有统计学意义（P=0．01）。结论FBXW7mRNA及其蛋白在食管鳞癌组织和食管高级别上皮内瘤变组织中的表达显著减低，且其低表达与食管鳞癌患者的不良预后密切相关。作为一种肿瘤抑制基因，FBXW7可能对食管鳞癌的发生、发展和转移起重要作用，有可能成为反映食管鳞癌患者病情严重程度及判断其预后的重要分子生物学标志物。
Objective To evaluate the expression of FBXW7 in esophageal squamous cell carcinoma （ESCC） and to explore the correlation of FBXW7 expression with clinicopathologic features and prognosis of ESCC. Methods Ninety cases of ESCC and twenty cases of tumor-adjacent normal tissues and forty intraepithelial neoplasia tissues were detected by immunohistochemistry methods. The expression of FBXW7 in 40 surgical ESCC tissues and tumor-adjacent normal tissues was detected by Western blotting and RT-PCR. The relationship between the expression of FBXWT, clinicopathological characteristics and prognosis was analyzed. Results The positive rate of FBXW7 protein was significantly lower in the ESCC and high-grade intraepithelial neoplasia tissues than in the tumor-adjacent normal tissues and low grade intraepithelial neoplasia tissues （40.0% and 33.3% vs. 85.0% and 77.3%, χ^2= 21.923, P〈0.001） . The FBXW7 protein was down-regulated in ESCC tissues （P〈 0.05 ）, whereas the FBXW7 mRNA was down- regulated in ESCC tissues compared with that in the paracancerous tissues. The expression of FBXW7 was significantly correlated with TNM stage, degree of differentiation, invasion depth and lymph node metastasis χ^2=9.643, 14.908, 16.294, 10.222, respectively, P=0. 002, 0.001, 0.000, 0.001）. In the ninety patients, the 5-year survival rates of cases with positive and negative expression of FBXW7 protein was 67.6% and 39.3%, respectively （χ^2 = 6.699, P = 0.01 ）. Conclusions The results of this study demonstrate that FBXW7 expression is significantly declined in ESCC and high grade intraepithelial neoplasia tissues, and is closely correlated with poor prognosis of this disease. FBXW7 as a tumor suppressor gene may play an important role in the carcinogenesis, development and metastasis of ESCC. These results suggest that FBXW7 may become a valuable marker for the severity and prognosis in ESCC.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2015年第5期347-351,共5页
Chinese Journal of Oncology
基金
国家自然科学基金（30770991）
安徽省铜陵市科技计划资助项目（201311）
关键词
食管肿瘤
F框/WD-40域蛋白7
免疫组织化学
逆转录聚合酶链反应
预后
Esophageal neoplasms
F-box and WD repeat domain-containing domain protein7
Immunohistochemistry
Reverse transcriptase-polymerase chain reaction
Prognosis
