摘要
目的:评价包载PTH(1-34)聚乳酸微球的缓释效应对前成骨细胞MC3T3-E1成骨分化的影响。方法:将MC3T3-E1细胞分为空白培养液对照组、持续性和间歇性给药组、未载药PLGA微球组和载药PLGA微球组。通过碱性磷酸酶(ALP)染色、茜素红染色和Reverse Transcription PCR观察负载PTH(1-34)的PLGA微球对细胞成骨分化的影响。结果:释放终浓度为10-9mol/L的载药PLGA微球组可以提高成骨细胞ALP活性,促进矿化,上调RUNX2、ALP和VEGF基因的表达。结论:PLGA微球缓释PTH(1-34)可促进成骨细胞分化。
Objective：To determin the effect of PLGA microspheres loading with PTH（1-34）[PTH（1 34）/PLGA]on the differentiation of MC3T3E1 cells.Methods：MC3T3E1 cells were divided into control group,continuous or intermittent PTH（1-34）adminstration groups,PLGA microsphere group and PTH（1-34）/PLGA group.Osteogenesis differentiation was observed by alkaline phosphatase activity（ALP）,alizarin red staining and RTPCR.Results：The PTH（1 34）/PLGA with 10-9 mol/L final release concentration enhanced ALP activity and mineralization,increased the mRNA expression of RUNX2,ALP and VEGF.Conclusion：Controlledrelease of PTH（1-34）from PLGA microspheres can promote the osteogenesis differentiation of MC3T3E1 cells.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2015年第3期360-364,共5页
Journal of Practical Stomatology
基金
吉林省产业技术研究与开发专项项目(编号:2013C022-6)
