摘要
目的制备肾炎滴丸并考察其药效学。方法正交实验优化肾炎滴丸的制备工艺;采用切除5/6肾的方法建立大鼠慢性肾衰竭模型,考察肾炎滴丸对慢性肾衰竭大鼠尿蛋白及肾脏组织结构的影响。结果肾炎滴丸的最佳制备工艺为PEG4000∶PEG6000=2∶1,药物∶基质=1∶3,滴制温度80℃,滴距7 cm;药效学结果显示:与模型对照组比较,肾炎滴丸组与肾衰丸组大鼠24 h尿蛋白水平均明显降低(P<0.01),肾炎滴丸组降低更为显著(P<0.05);病理组织检查结果显示治疗组大鼠肾小管基本恢复正常,间质有少量炎症细胞浸润,肾间质纤维组织增生得到较好抑制。结论肾炎滴丸制备工艺稳定,且对慢性肾衰竭有较好的作用,但最佳治疗剂量还有待进一步验证。
Objective To prepare nephritis dripping pills and study its pharmacodynamic effects. Methods The nephritis dripping pills were self-made, and the preparation was optimized by using orthogonal method. The model of chronic renal failure was established through resection of 5/6 kidney, the effect of nephritis dripping pills on rats was investigated by urinary protein and renal pathology analysis. Results The optimal preparation for nephritis dripping pills was as follows：taking PEG4000：PEG6000 =2：1, drug：matrix =1：3, setting temperature at 80 ℃ and dropping distance as 7 cm. The pharmacodynamic results showed that：compared with the model control groups, the nephritis dropping pills and renal failure pills significantly reduced the 24 h urine protein levels （P〈0. 01）,and the nephritis dripping pills were significant superior to renal failure pills （P〈0. 05）. The histopathological results showed that the renal tubular of treated groups remitted to normal, renal interstitial presented a small amount of inflammation cell infiltration and renal interstitial fibrosis was suppressed. Conclusion The preparation of nephritis dripping pills is relatively stable,and have good therapeutic effects on chronic renal failure,but the optimal dose should be further verified.
出处
《医药导报》
CAS
2015年第5期617-620,共4页
Herald of Medicine
关键词
肾炎滴丸
肾衰竭
慢性
尿蛋白
Nephritis dripping pill
Renal failure,chronic
Urinary protein
