摘要
背景:肺腺癌干细胞是肺腺癌确诊的重要实验室依据,但其评估患者预后的价值如何,临床尚未定论。目的:探讨人肺腺癌干细胞表型与患者预后的关系。方法:选取2010年2月至2013年1月在新疆医科大学第一附属医院治疗的肺腺癌患者48例,采用免疫荧光法检测患者肺腺癌干细胞表型标志,比较不同表型与患者临床病例特征以及患者预后的关系。结果与结论:48例肺腺癌组织中均观察到SP-C表达和CCSP表达,具有肺细支气管肺泡干细胞的表型特征,其中34例同时表达OCT4(OCT4+支气管肺泡干细胞组),14例不表达OCT4(OCT4-支气管肺泡干细胞组)。OCT4+支气管肺泡干细胞组和OCT4-支气管肺泡干细胞组肺腺癌患者的年龄、性别、吸烟史以及肿瘤分期差异无显著性意义(P>0.05);OCT4+支气管肺泡干细胞组有23例(68%)患者癌细胞转移,明显高于OCT4-支气管肺泡干细胞组(21%),差异有显著性意义(P<0.05);在腺癌患者年龄、性别、吸烟史、分期以及癌细胞转移等临床病理特征分层中,OCT4-支气管肺泡干细胞组肺腺癌2年生存率普遍高于OCT4+支气管肺泡干细胞组,两组生存曲线差异有显著性意义(P<0.05)。结果表明人肺腺癌干细胞具有肺细支气管肺泡干细胞表型特征,同时表达胚胎干细胞基因OCT4时,患者预后差,与肺腺癌转移有关。
BACKGROUND： Lung adenocarcinoma stem cells are a very important marker for diagnosis of lung adenocarcinoma.OBJECTIVE： To investigate the relationship between human lung adenocarcinoma stem cell phenotypes and patient＇s prognosis.METHODS： From February 2010 to January 2013, 48 patients with lung adenocarcinoma admitted in the First Affiliated Hospital of Xinjiang Medical University were enrolled. Lung adenocarcinoma cancer stem cell phenotypes were detected with immunofluorescence method, and the relationship of different phenotypes and clinical characteristics with patient＇s prognosis was compared.RESULTS AND CONCLUSION： SP-C and CCSP expression was observed in the lung adenocarcinoma tissues of48 cases, possessing the phenotypic characteristics of bronchioloalveolar stem cells. Of the 48 patients, OCT4 was positive in 34 cases（OCT4^＋ group） and negative in 14 cases（OCT4-group）. There was no difference between the two groups in terms of patient＇s age, gender, smoking history and tumor stage（P〉0.05）. There were 23 cases（68%）in the OCT4^＋ group with cancer cell metastasis, which was significantly higher than that in OCT4-group（21%; P〉0.05）. In the aspects of patient＇s age, gender, smoking history, staging and cancer metastasis and other clinical pathological stratification, the 2-year survival rate in the OCT4-group were generally higher than that in the OCT4^＋group, and there was a significant difference in the survival curves of two groups（P〈0.05）. Human lungadenocarcinoma stem cells have the phenotypic characteristics of bronchioloalveolar stem cells, and meanwhile, if the cells are positive for OCT4, patient＇s prognosis is poor and associated with lung adenocarcinoma metastasis.
出处
《中国组织工程研究》
CAS
北大核心
2015年第10期1523-1527,共5页
Chinese Journal of Tissue Engineering Research
