摘要
目的：探讨富血小板血浆（ PRP）体外抑菌作用。方法制备富血小板血浆（ PPP）、乏血小板血浆,以LB液体培养基作为阴性对照,观察37℃及22℃条件下,PRP、PPP分别在不同时间段（2h、4h、6h、8h、12h、24h）对金黄色葡萄球菌、表皮葡萄球菌、铜绿假单胞菌是否有抑菌作用。结果37℃及22℃条件下,PRP、PPP组对金黄色葡萄球菌和表皮葡萄球菌生长的均显示出较好的抑菌效果,并具有时效性。对于金黄色葡萄球菌,最大抑菌作用出现在2~4h；4h后,PPP抑菌效果减弱,而PRP则继续维持较好的抑菌作用直到12h；24h后,两者均无抑菌效果。 PRP和PPP组对表皮葡萄球菌抑菌作用则可持续到24h。血小板浓度高低对抑菌效果的影响差别不大。 PPP和PRP组对铜绿假单胞菌未显示出抑菌效果。结论富血小板血浆具有特定的抑菌谱,且对不同菌属的抑菌作用具有时效性。
Objective To study the antibacterial effects of platelet rich plasma in vitro. Methods Prepare platelet-rich plasma（ PRP） , platelet-poor plasma（ PPP） , Use the liquid LB medium as negative control,observe the antibacterial effect of PRP and PPP under the condition of 37 ℃ and 22 ℃ respectively at different times （2h, 4h, 6h, 8h, 12h, 24h）against Staphylococ-cus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa. Results Under the condition of 37 ℃ and 22 ℃,both PRP and PPP showed good antibacterial effects on the growth of Staphylococcus aureus and Staphylococcus epidermidis, which was time-bound. For Staphylococcus aureus, the maximum bacteriostatic effect appeared during 2 ~ 4h;the bacteriostatic effect of PPP decreased after 4h, while PRP maintained the good bacteriostatic effect which could sustainable to 12h;neither PRP or PPP had antibacterial effect 24h later. The bacteriostatic effect of PRP and PPP against Staphylococcus epidermidis could sustainable to 24h. There was no significant relationship between the concentration of platelet and antimicrobial effect. Either PPP or PRP showed no antibacterial activity against Pseudomonas aeruginosa. Conclusion Platelet rich plasma has specific antibacterial spectrum, and the bacteriostatic effect of different bacterial species is time-bound.
出处
《四川医学》
CAS
2015年第4期470-473,共4页
Sichuan Medical Journal
基金
四川省卫生厅科研课题(编号:110474)
关键词
富血小板血浆
抑菌作用
platelet rich plasma
bacteriostasis
