摘要
目的分析该院2013年临床分离病原菌的分布及耐药性,为临床正确诊断和合理使用抗菌药物提供依据。方法采用MicroScan Walk Away 40SI全自动细菌鉴定/药敏分析仪对菌株进行鉴定和药敏试验,并运用WHONET5.4进行结果分析。结果从13 379例标本中共分离病原菌3 701株,其中革兰阳性菌776株占21.0%,革兰阴性菌2 192株占59.2%,排名前5位病原菌依次为大肠希氏菌、肺炎克雷伯菌、铜绿假单胞菌、鲍曼不动杆菌、金黄色葡萄球菌,分别占18.7%、18.1%、13.2%、9.2%、9.3%。大肠埃希氏菌和克雷伯菌属产超广谱β内酰胺酶检出率分别为51.0%和26.7%。耐甲氧西林凝固酶阴性葡萄球菌和耐甲氧西林金黄色葡萄球菌检出率分别为79.5%和43.0%。肠杆菌科细菌对碳青霉烯类保持较高敏感性,革兰阳性菌对万古霉素和利奈唑胺均敏感。结论必须加强细菌耐药性监测,指导合理用药。
Objective To explore the distribution and drug resistance of clinical isolated pathogens so as to provide scientific ba- sis for the correct diagnosis and ration use of antibiotics. MethodsThe species identification and antibiotic resistance tests were per- formed with the MicroScan Walk Away 40 SI, and then the result were analyzed with WHONET5.4. Results A total of 3 701 strains of pathogens were isolated from 13 379 specimens, among which there were 776 （21.0%） strains of them were gram-positive cocci and 2 192 （59.2%） strains of gram negative bacilli; The Escherichia coli, Klebsiella pueumoniae, Pseudomonas aerugi- nosa, Acinetobacter baumannii and Staphylococcus aureus ranked the top five position of pathogens, accounting for 18. 7%, 18.1 %, 13.2 %, 9.2 %, 9.3 %. 51.0 % of the Escherichia coil and 26.7 % of Klebsiella pueumoniae were tested positive of extended spectrum/3-1actamase. The detection rates of methicillin resistant coagulase-negaive Staphylicocci and methcillin-resistant Staphylo- coccus aureus were 79.5% and 43.0%. Enterobaeteriaceae remained at a high sensitivity to carbapenems, and gram-positive cocci were sensitive to vaneomycin and linezolid. Conclusion Drug resistance monitoring should be strengthened in order to instruct ra- tional use of antibiotoces.
出处
《国际检验医学杂志》
CAS
2015年第9期1210-1212,共3页
International Journal of Laboratory Medicine
关键词
医院感染
病原菌
分布
耐药性
hospital infection
pathogenic bacteria
distribution
drug resistance
