摘要
目的研究胰高糖素样肽-1受体激动剂艾塞那肽对2型糖尿病大鼠糖脂代谢和胰岛细胞的影响及其作用机制。方法 健康雄性SD大鼠随机分为正常对照组(C组6只)、正常对照+艾塞那肽处理组(C+E组6只)、糖尿病组(D组5只)以及糖尿病+艾塞那肽处理组(D+E组5只),由高脂饮食加小剂量STZ诱导成2型糖尿病后,D+E组以及C+E组大鼠予艾塞那肽,5μg,腹部皮下注射,1次/d干预;8周后,分别测定各组大鼠体重、FBG、空腹胰岛素(FINS)、稳态模型胰岛素抵抗指数(HOMA-IR)、胰岛素敏感指数(ISI)以及血脂谱。同时行透射电子显微镜观察大鼠胰岛组织超微结构改变。结果 D+E组大鼠FBG、FINS、TG、TC、LDL、HOMA-IR水平均明显低于未予干预的D组大鼠(均P<0.05),ISI则显著高于D组大鼠(P<0.05)。超微结构研究,与D组大鼠比较,D+E组大鼠胰岛β细胞数量增加,形态较规则,胞质分泌颗粒增加,颗粒密度较正常;仅见少量线粒体肿胀,结构模糊。内质网结构无明显破坏。结论艾塞那肽能显著改善2型糖尿病大鼠糖脂代谢紊乱,对其残存的胰岛细胞具有明确的保护作用。
Objective To investigate the effects of glucagon- like peptide- 1 agonist exenatide on glucose and lipid metabolism and ultrastructure of islet cel s in type 2 diabetic rats. Methods Twenty- two male SD rats were randomly divided in-to non- diabetic control group （group C, n=6）;non- diabetic＋exenatide group （group C＋E, n=6）;diabetic group （group D, n=5） and diabetic＋exenatide group （group D＋E, n=5）. Type 2 diabetes was induced by high- fat diet and low- dose streptozotocin in rats. The rats in C＋E group and D＋E group were treated with exenatide （5μg/d ） by subcutaneous injection, while the rats in C group and D group were injected with the same volume of normal saline. After 8 weeks, the body weight, fasting blood glucose, insulin, homeoestasis model assessment （HOMA- IR）, insulin sensitivity index （ISI） and blood lipid profile were determined in al groups. The ultrastructure of islet cel s was observed with transmission electron microscopy. The one- way ANOVAs fol owed by a Student- Newman- Keuls post hoc test for multiple comparisons were used for statistics. Results Compared with group D, the fasting blood glucose, insulin, triglyceride, total cholesterol, lower density lipoprotein levels, and HOMA- IR were significantly de-creased （al P〈0.05）, while ISI significantly increased （P〈0.05） in the D＋E group. Electron microscopy observation revealed that exenatide attenuated the swol en mitochondria and endoplasmic reticulum in islet cel s of the diabetic rats. Conclusion The glucagon- like peptide- 1 agonist exenatide can significantly improve glucose and lipid metabolism and protect islet cel s in type 2 diabetic rats.
出处
《浙江医学》
CAS
2015年第5期363-366,共4页
Zhejiang Medical Journal
基金
浙江省中医药优秀青年人才基金项目(2013ZQ004)
