摘要
针对盐度胁迫下硝化微生物富集难、亚硝酸盐易积累等问题，以亚硝态氮为唯一氮源，开展了不同盐度（NaCl）对序批式移动床生物膜驯化前后硝化特性影响的研究。结果表明，随着驯化盐度的提升（R1-R4的盐度分别为0、10、20、40g／L），硝化生物膜成熟时间逐渐延长，分别为31、31、39及〉50d，反应器R1～R3性能稳定时出水NO2--N浓度〈0．1mg／L。采用非竞争性抑制模型获得不同反应器内盐度对硝化性能的半抑制浓度分别为〈40、44．36和62．94g／L，表明在0-20g／L盐度范围内，驯化盐度越高则生物膜的耐盐冲击性能越强。R2和R3中生物膜的EPS含量为147．91和102．45mg／gVS，明显高于R1（94．61mg/gVS）。DGGE结果显示，四组生物膜微生物种群结构丰富，对照组R1与R2、R3、R4的相似性系数分别为68．7％、66．0％和44．7％，反映出微生物群落结构相似性随盐度差异增大而明显降低。相比较而言，当盐度≤20g／L时，硝化生物膜反应器可直接启动；盐度〉20g／L时，可考虑梯度盐度驯化法启动反应器，以20g／L盐度为基准，可在一个月左右获得成熟的硝化生物膜并使其获得较高的耐高盐冲击能力。
Due to the problems of difficult enrichment of nitrifying microorganisms and facile accu- mulation of nitrite under salinity stress, the effects of different salinities （NaC1） on nitrification perform- ance of sequencing batch moving bed biofilm reactors before and after acclimation were investigated using the nitrite as the sole nitrogen source. The were gradually increased up to 31 d, 31 d, to R4 with a salinity of 0, 10, 20 and 40 the reactors R1 to R3 were all less than 0. results showed that the maturation periods of nitrifying biofilm 39 d and 〉 50 d with the increase of acclimation salinity （ R1 g/L respectively）. The effluent concentrations of NO2- -N in 1 mg/L when the biofim maturation was achieved. The semi- inhibitory concentrations of nitrification under the different salinity circumstances were obtained using the noncompetitive inhibition model, which were 〈 40 g/L （ R1 ）, 44.36 g/L （R2） and 62.94 g/L （ R3 ）,respectively. It was indicated that the higher the salinity was within the range of 0 to 20 g/L, the stronger the salinity tolerance of nitrifying biofilm would be. The analysis of extracellular polymeric substances （EPS） demonstrated that the EPS contents in biofilm reactors R2 （147.91 mg/gVS） and R3 （102.45 mg/gVS） were both much higher than that in R1 （94.61 mg/gVS）. The results of DGGE fingerprinting further proved that the microbial community structure of biofilm in four groups was rich, and the similarity coefficients of R1 with R2, R3 and R4 were 68.7% 66.0% and 44.7%, indicating that the similarity of microbial community structure was significantly reduced with the increase of salinity difference. It might be concluded that nitrifying biofilm reactor could be started up directly at a salinity of less than or equal to 20 g/L. When the salinity was greater than 20 g/L, the salinity gradient acclimatization way could be considered, and the mature nitrification biofilm with a high resistance to high salt shock would be obtained in a month.
出处
《中国给水排水》
CAS
CSCD
北大核心
2015年第9期6-10,共5页
China Water & Wastewater
基金
国家科技支撑计划项目(2012BAJ25B07)
浙江海洋学院科研启动项目(21105012313)
浙江省新苗人才计划项目(2014R411024)
关键词
盐度
移动床生物膜
亚硝态氮
硝化
salinity
moving bed biofilm
nitrite
nitrification
