摘要
以苘麻成株叶片为材料,采用植物抗性生理分析测定技术研究了淡紫灰链霉菌除草活性物质D-13组分对苘麻叶片细胞膜透性、叶片内MAD含量、SOD、CAT和APX酶活性变化的影响,以及对膜系统的影响。结果表明,苘麻叶片经D-13组分处理后,细胞膜透性随着D-13组分浓度的增加而增加,MDA含量在D-13组分处理后,随着D-13组分浓度的增加而升高。在电镜观察下,叶片细胞的膜系统随着D-13组分浓度的升高呈现出不同的受害情况。随着D-13组分处理时间的延长,SOD、CAT、APX活性均出现了不同程度的下降,与对照相比,SOD、CAT、APX在40h时活性达到最低,分别比对照活性降低超过了60%、30%和50%。可见,淡紫灰链霉菌除草活性物质可严重降低苘麻叶片的生理生化活性。
The effects of the substances with herbicidal activity,D-13 components,fromStreptomyces lavendulae on membrane permeability,MDA content,SOD,CAT and APX activities in cells of Abutilon theophrasti Medicus leaves were studied through analyzing the resistance physiology.The results showed that the cell membrane permeability and the MDA content increased with the increase of D-13 concentration.Under the electron microscope,leaf cell membrane system showed different symptoms with the increase of D-13 concentration.SOD,CAT and APX activities declined to different degrees with the extension of processing time of D-13 components.Compared with the controls,SOD,CAT and AXP activities were the lowest at 40 h,decreasing more than60%,30%and 50%,respectively,compared with the control.It is concluded that D-13 components could effectively depressed the physiological activities of A.theophrasti leaves.
出处
《植物保护》
CAS
CSCD
北大核心
2015年第2期24-28,48,共6页
Plant Protection
基金
公益性行业(农业)科研专项(201303031)
关键词
除草活性物质
膜透性
MDA
酶活性
超微结构
substance with herbicidal activity
membrane permeability
MDA
enzyme activity
ultrami-crostructure
