摘要
目的探讨断乳至性成熟期经口持续摄入纳米二氧化硅对小鼠卵巢发育的影响。方法将40只21日龄清洁级ICR雌性小鼠随机分为4组,即对照组和0.25、0.50、1.00 g/kg纳米二氧化硅染毒组,每组10只。采用经口灌胃方式进行染毒,每日1次,持续染毒5周。实验结束后记录小鼠体重增长情况、观察阴门开放时间和动情周期,病理切片计数各级卵泡数量及计算各级卵泡的构成比,并测定卵巢中丙二醛(MDA)含量和超氧化歧化酶(SOD)、谷胱苷肽过氧化物酶(GSH-Px)活力。结果与对照组比较,1.00 g/kg纳米二氧化硅染毒组小鼠卵巢重量和脏器系数下降、阴门开放时间推迟、卵巢黄体构成比下降、闭锁卵泡构成比升高,差异有统计学意义(P<0.05);而各剂量纳米二氧化硅染毒组小鼠体重增长和动情周期均无明显改变。与对照组比较,1.00 g/kg纳米二氧化硅染毒组小鼠卵巢组织中MDA含量明显上升,SOD活力均明显下降,而0.50、1.00 g/kg纳米二氧化硅染毒组小鼠卵巢组织中GSH-Px活力均升高,差异具有统计学意义(P<0.05)。结论经口摄入纳米二氧化硅可影响小鼠卵巢发育,其机制可能与氧化损伤有关。
Objective To study the effects of nano-SiO2 oral exposure on ovarian development in mice from weaning to sexual maturity. Methods Forty clean grade ICR mice [postnatal day （PND） 21] were randomly divided into control,0.25,0.50 and 1.00 g/kg body weight nano-SiO2 groups. The mice were treated with nano-SiO2 at the doses of 0.25,0.50 and 1.00 g/kg body weight, respectively, through gavage once a day for five consecutive weeks. After the treatment,the body weight gain was recorded, the day of vagina opening and the estrous cycle were observed,the ovarian coefficient and the constituent ratio of different stage follicles were calculated, the levels of MDA, SOD and GSH-Px of ovary were determined. Results The ovarian coefficient of the 1.00 g/kg body weight nano-SiO2 group was lower than that of the control group （P〈0.05）. Compared with control group,the age of vagina opening in the 1.00 g/kg nano-SiO： group was significantly delayed （P〈0.05）. As for the body weight gain and the estrous cycle, there were no significant differences among all groups. Compared with the control group, the constituent ratio of corpus luteum in 1.00 g/kg body weight nano-SiO2 group decreased and that of atretic follicles increased significantly （P〈0.05）. Compared with control group,the MDA level of ovary in the 1.00 g/kg body weight nano-SiO2 group increased and that of SOD enzyme activity decreased significantly （P〈0.05）. However,the GSH-Px enzyme activity of ovary in the 0.50 and 1.00 g/kg body weight nano-SiO2 groups increased significantly （P〈0.05）. Conclusion Oral exposure to nano-SiO2 may have adverse effect on ovarian development and the mechanism may relate to oxidative damage induced by nano-SiO2.
出处
《环境与健康杂志》
CAS
北大核心
2015年第1期22-25,F0003,共5页
Journal of Environment and Health
基金
福建医科大学"环境与健康重点实验室"新技术研究与应用项目(201407)
关键词
纳米二氧化硅
卵巢
丙二醛
Nano-silicon dioxide
Ovary
Malondialdehyde
