摘要
目的:研究miR-450a-5p对体外培养的人浆液性卵巢癌SKOV3细胞增殖、迁移、侵袭、周期、凋亡机制的影响,分析miR-450a-5p对人卵巢癌SKOV3细胞肿瘤生物学行为的影响。方法:运用Hiperfect转染试剂介导的转染法将miR-450a-5p mimics转染人卵巢癌SKOV3细胞,设阴性对照组(NC组)及空白对照组(blank组)。采用MTT法、Transwell迁移及侵袭实验、划痕实验、流式细胞术,分别检测细胞增殖、迁移及侵袭能力、周期及凋亡。结果:MTT实验:miR-450a-5p mimics组细胞增殖活性与NC组、blank组无明显差异(P>0.05)。Transwell迁移及侵袭实验均显示转染miR-450a-5p mimics后,细胞的迁移及侵袭能力明显减弱(P<0.05)。划痕后miR-450a-5p mimics组细胞迁移能力较NC组、blank组降低(P<0.05)。流式细胞术:转染miR-450a-5p mimics后,mimics组细胞周期明显被阻滞于G1期(P<0.05)。miR-450a-5p mimics组、NC组、blank组凋亡结果无明显差异(P>0.05)。结论:过表达miR-450a-5p可能对人卵巢癌细胞系SKOV3的侵袭及迁移能力存在负性调控,可能将SKOV3细胞周期阻滞于G1期。
Objective： To investigate the effect of miR- 450a- 5p on proliferation,migration,invasion,cell cycle and apoptosis in SKOV3 cells and explore its effect on the biological behavior of serous ovarian cancer SKOV3 cells.Methods： SKOV3 cells were transfected with miR- 450a- 5p mimics by hiperfect transfection reagents as experimental group compared to negative control group（ NC group） and blank group. Cell proliferation ability was evaluated by MTT assay. Cell migration ability was detected by transwell migration assay and wound healing assay. Cell invasion ability was detected by transwell invasion assay. Cell cycle and apoptosis were detected by flow cytometry. Results：There were no significant differences between mimics group and control groups by MTT assay（ P〈0. 05）. Transfection of miR- 450a- 5p mimics into SKOV3 cells led to significant inhibition effect on cell migration and invasion ability detected by transwell assay（ P〈0. 05）. The migration ability of mimics group was significantly inhibited（ P〈0. 05）. Flow cytometry analysis displayed that miR- 4 5 0 a- 5 p significantly induced SKOV3 cell G1arrest（ P〈0. 05）,but no significant differences were found in cell apoptosis（ P〈0. 05）. Conclusion： miR- 450a- 5p overexpression may negatively regulate the invasion and migration ability of SKOV3 cells and may induce SKOV3 cells G1 arrest.
出处
《现代肿瘤医学》
CAS
2015年第7期892-896,共5页
Journal of Modern Oncology
基金
国家重大基础研究计划(973计划)(编号:2015CB553703)
卫生部医药科技发展研究中心课题(编号:W2013FZ13)
陕西省社会发展攻关计划项目(编号:2014K11-01-01-25)
