摘要
目的探讨胃癌组织中转化生长因子β1(TGF-β1)、Smad2与细胞周期蛋白D1(CyclinD1)的表达及三者之间的相关性。方法选择2007年1月至2012年1月腔镜活检正常胃黏膜20份(正常胃组织)作为对照组,手术切除胃癌标本60份(高-中分化、低分化)作为胃癌组,用免疫组化法检测TGF-β1、Smad2、CyclinD1正常胃组织及胃癌组织中的表达。结果 TGF-β1及CyclinD1在胃癌组织中的表达高于对照组,Smad2在胃癌组中的表达低于对照组;TGF-β1及CyclinD1在低分化胃癌组中的表达高于高-中分化胃癌组;TGF-β1及CyclinD1在无淋巴结转移的胃癌组中的表达低于有淋巴结转移的胃癌组;TGF-β1、CyclinD1在胃癌组中的表达随着TNM分期的提高而升高,高-中分化胃癌组Smad2较低分化胃癌组表达升高,Smad2的表达在无淋巴结转移的胃癌组中的表达高于有淋巴结转移的胃癌组;Smad2在胃癌中的表达随TNM分期的提高而降低,TGF-β1、Smad2及CyclinD1的表达与患病年龄、性别无关;TGF-β1与Smad2呈负相关,TGF-β1与CyclinD1呈正相关,CyclinD1与Smad2呈负相关。结论 TGF-β1、Smad2与CyclinD1的表达与胃癌的发病有关。
Objective To explore the expression of TGF‐β1 ,Smad2 and CyclinD1 protein in gastric carcinoma tissue and their correlation .Methods 20 specimens of normal gastric tissue by the endoscopic biopsy （control group） and 60 surgical specimens （high‐middle differentiation ,low differentiation） of gastric carcinoma（gastric cancer group） were collected from January 2007 to January 2012 .The immunohistochemical method was used to detect the expression of TGF‐β1 ,Smad2 and CyclinD1 in gastric cancer tissues and normal gastric mucosal tissues .Results The expression of TGF‐β1 and CyclinD1 in the gastric cancer group was signifi‐cantly higher than that in the normal gastric tissue group ,while the expression of Smad2 in the gastric cancer group was significant‐ly lower than that in the normal gastric tissue group ;the expression of TGF‐β1 and CyclinD1 in the low differentiation group was higher than that in the high‐middle differentiation group ,the expression of TGF‐β1 and CyclinD1 in the non lymph node metastasis group was lower than that in the lymph node metastasis group ;the expression of TGF‐β1 and CyclinD1 protein in gastric cancer was increased with the increase of the TNM staging .The expression of Smad2 in the high‐middle differentiation group was higher than that in the low differentiation group ,the expression of Smad2 in the non‐lymph node metastasis group was higher than that in the lymph node metastasis group ;the expression of Smad2 in gastric cancer was decreased with the increase of the TNM staging .The expression of TGF‐β1 ,Smad2 and CyclinD1 in the gastric cancer group had no relation with age and gender .TGF‐β1 was negatively correlated with Smad2 ,TGF‐β1 was positively correlated with CyclinD 1 ,CyclinD1 was negatively correlated with Smad2 .Conclusion TGF‐β1 and Smad2 are related with the CyclinD1 expression and pathogenesis of gastirc carcinoma .
出处
《重庆医学》
CAS
北大核心
2015年第11期1481-1483,1486,共4页
Chongqing medicine
基金
河北省医学科学研究重点课题计划资助项目(ZL20140103)
