摘要
目的:比较紫外分光光度法和高效液相色谱法测定合欢花总黄酮含量的差异,探讨合欢花总黄酮含量测定方法的可靠性。方法:紫外分光光度发以槲皮苷为指标性成分,在435nm波长处进行测定。高效液相色谱法以槲皮苷计总黄酮含量。结果:紫外分光光度法测得的合欢花总黄酮含量以槲皮苷计为29.8%。高效液相色谱法测得的合欢花总黄酮含量以槲皮苷计为28.2%。结论:紫外分光光度法和高效液相色谱法测定的合欢花总黄酮含量差异较小,紫外分光光度法可以代替高效液相色谱法对总黄酮进行快速测定。
Objective ： To investigate the quantitative determination of total flavonoids from the flowers of Albiz- zia julibrissin Durazz. by using UV spectrophotometry and HPLC. Methods： Quercitrin was used as the refer- ence compound for UV spectrophotometry, and it was determined at 435nm. HPLC was performed by using Quercitrin as the reference compound. Results ： The content of total flavonoids from the flowers of A. julibris- sin Durazz. was 29.8% by UV spectrophotometry, but 28.2% by HPLC analysis. Conclusion： The contents of total flavonoids from the flowers of A. julibrissin Durazz. determined by UV spectrophotometry and HPLC have small difference, which suggests that both methods are suitable to determine the total flavonoids from the flow- ers of A. julibrissin Durazz.
出处
《中医药信息》
2015年第2期54-57,共4页
Information on Traditional Chinese Medicine
基金
北京中医药大学研究生自主研究课题(No.2013-JYBZZ-XS-100)
关键词
合欢花
总黄酮
紫外分光光度法
高效液相色谱法
Flowers of Albizzia julibrissin Durazz.
Total flavonoids
UV spectrophotometry
HPLC
