摘要
目的探讨凋亡抑制蛋白c-IAP1和促凋亡蛋白Smac在儿童急性白血病(AL)患者中的表达情况,分析二者之间的关系及临床意义。方法 AL患儿72例,其中初诊组32例,缓解组30例,复发组10例;另取同期12例非恶性血液病患儿作为对照组。应用免疫组织化学过氧化物酶标记链霉卵白素染色法分别检测患儿骨髓中c-IAP1、Smac蛋白表达情况;对其中45例患儿采用半定量反转录聚合酶链反应检测其骨髓单个核细胞中c-IAP1、Smac mRNA表达水平。结果 c-IAP1、Smac蛋白在初诊组AL患儿骨髓中表达水平均高于缓解组和对照组(H=19.345、16.621,P<0.008);复发组AL患儿c-IAP1、Smac蛋白表达水平高于对照组(H=8.324、8.324,P<0.008)。c-IAP1、Smac mRNA在初诊组AL患儿骨髓中表达水平均高于缓解组和对照组(H=7.973、5.942,P<0.017)。初诊组AL患儿骨髓中c-IAP1、Smac蛋白和mRNA水平在急性淋巴细胞白血病和急性髓细胞白血病间比较差异无统计学意义(t=-0.482、0.818,P>0.05)。初诊组AL患儿骨髓中c-IAP1、Smac蛋白及mRNA水平呈正相关(r=0.541、0.668,P<0.01)。结论c-IAP1、Smac蛋白参与了儿童AL的发生和发展,并且二者相互影响,但在初诊患儿中二者表达水平与白血病临床类型可能无关。
Objective To investigate the expression and clinical significance of cellular inhibitor of apoptosis（ c-IAP1）and second mitochondria-derived activator of caspases（ Smac） in children with acute leukemia（ AL）. Methods Bone marrow samples were obtained from 72 children with AL including 32 newly diagnosed children（ newly diagnosed group）,30 complete remission children（ complete remission group） and 10 relapsed children（ relapse group） and 12 children without malignant blood disease（ control group）. The expression of c-IAP1 and Smac protein were measured by streptavidin / peroxidase immunoperoxidase technique in all children,and the mRNA of c-IAP1 and Smac in bone marrow mononuclear cells of 45 cases were measured by semi-quantity reverse transcription-polymerase chain reaction. Results The expression levels of c-IAP1 and Smac protein in the bone marrow of newly diagnosed group were higher than those in complete remission group and control group（ H = 19. 345,16. 621; P〈0. 008）,and the expression levels of c-IAP1 and Smac protein in relapse group were higher than those in control group（ H = 8. 324,8. 324; P〈0. 008）. The mRNA levels of c-IAP1 and Smac in the bone marrow of newly diagnosed group were higher than those in complete remission group and control group（ P〈0. 017）. In newly diagnosed group,there was no statistical significance in the protein and mRNA expression of c-IAP1 and Smac between acute lymphoblastic leukemia and acute myelocytic leukemia（ t = 0. 482,0. 818; P〉0. 05）. In newly diagnosed group,the protein and mRNA levels of c-IAP1 were positive correlated with those of Smac（ r = 0. 541,0. 668; P〈0. 01）. Conclusion c-IAP1 and Smac protein may participate in and affect each other in the pathogenesis and progression of AL,but the levels of c-IAP1 and Smac may be not correlated with the types of AL.
出处
《新乡医学院学报》
CAS
2015年第3期245-248,共4页
Journal of Xinxiang Medical University
基金
河南省卫生厅医学科技攻关计划项目(编号:200803077)
