摘要
目的进一步明确内毒素耐受时炎性相关细胞因子的变化。方法昆明鼠分组处置。建立内毒素(LPS)耐受鼠模型。ELISA方法测定血清肿瘤坏死因子α(TNF-α),白细胞介素10(IL-10)和白细胞介素17(IL-17)浓度。结果 LPS对照组血清TNF-α、IL-10和IL-17浓度分别为(2 261.49±1 090.28)pg/m L,(7 780.42±2 320.04)pg/m L,(27.28±13.76)pg/m L,较健康对照组显著增高。而内毒素耐受组相应细胞因子血清浓度分别为(1 174.19±450.91)pg/m L,(1 293.26±520.85)pg/m L,(22.26±11.44)pg/m L。地塞米松干预进一步降低了TNF-α和IL-17的水平,但对IL-10水平无显著影响。结论内毒素耐受状态与内毒素炎症状态比较,TNF-α和IL-10水平显著降低,IL-17水平无显著变化。应用地塞米松可进一步降低内毒素耐受状态下的TNF-α和IL-17水平。
Objective To further determine the inflammation-associated cytokine changes during endotoxin（LPS）tolerance. Methods Kun-ming mice were divided into groups. The endotoxin tolerance mouse model was established with repeated injection of LPS. Serum levels of tumor ne-crosis factor-α（TNF-α）,interleukin-10（IL-10）and IL-17 were determined by ELISA assay. Results The serum concentrations of TNF-α,IL-10 and IL-17 were 2 261.49±1 090.28 pg/mL,7 780.42±2 320.04 pg/mL,and 27.28±13.76 pg/mL in the LPS control group,which were increased significantly compared to the normal healthy control group. The serum concentrations of TNF-α,IL-10 and IL-17 were 1 174.19±450.91 pg/mL, 1 293.26±520.85 pg/mL,and 22.26±11.44 pg/mL in the endotoxin tolerance group. Levels of TNF-αand IL-17 were further decreased by dexameth-asone treatment during LPS tolerance inducing,while the IL-10 level did not change significantly. Conclusion Compared to the status of endotoxin inflammation,levels of TNF-αand IL-10 decreased significantly,while levels of IL-17 were not significantly changed in the status of endotoxin toler-ance. Dexamethasone could further suppress TNF-αand IL-17 levels during endotoxin tolerance inducing.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2015年第3期259-262,共4页
Journal of China Medical University
