摘要
以南天竹茎段为试材,采用正交设计对初代培养中基本培养基类型、BA浓度、NAA浓度、蔗糖浓度4个因素进行优化,旨在筛选最佳组合,以提高初代培养的诱导率。结果表明:4个因子对南天竹愈伤组织或芽的诱导影响程度依次为基本培养基类型、蔗糖浓度、BA浓度、NAA浓度;初代培养外植体的诱导最佳组合为MS+BA 1.0mg/L+NAA 0.2mg/L+20g/L蔗糖,诱导率达74.4%。
The regenerations of Nandina dornestica was investigated by using in vitro stem sections by the orthogonal experimental design. Stem sections were cultured on different basic medium supplemented different cx）ncentrations of benzyladenine （BA） in combination with different concentrations of mnaphthaleneacetic acid （NAA） and sugar, the experiment was to select the optimum combiration,to improve callus formation. The results showed significant differences in the percentage of callus formation were observed among the basic medium,sugar,BA and NAA. The highest percentage of callus formation （74. 4%） was obtained on .MS medium with 1.0 mg/L BA.0 9 mg/L NAA and 20g/L sugar.
出处
《北方园艺》
CAS
北大核心
2015年第5期93-96,共4页
Northern Horticulture
基金
湖北省自然科学基金资助项目(2009CDZ034)
湖北省高等学校青年教师深入企业行动计划资助项目(XD2012507)
关键词
南天竹
离体
初代培养
正交设计
诱导率
Nandina domestica Thunb
in vitro
primary culture
the orthogonal experimental design
the percentage of induction
