摘要
为了解决银耳栽培生产中存在菌株优良性状单一,生产效率较低等问题,采用银科1#和Tr21两个不同品种菌株为亲本菌株,通过单孢子分离选取50个有性孢子单核体、两两配对有性杂交试验,显微观察镜检鉴定,统计杂交成功率为36%、杂交菌株与亲本的拮抗试验选育出7株杂交株,栽培对比农艺性状评价选育出1株(GT3)比亲本菌株生长快,生物转化率较高的菌株。试验结果表明有性杂交育种可作为一种行之有效的筛选银耳优良菌株的方法。
During the process of Tremella fuciformis cultivation, some problems, such as low yield and long cultivation time, often are followed with different strains. The aim of this study is to mix good properties of different strain together to new strains by sexual hybridization method. Based comparison of yield, fresh ear ventral color and mature culture, yinke 1# and Tr21 were selected as parents strains with good properties. 50 single spores were isolated from fresh ear and partnered each other following single hybridization principle. 17 combinations are identified with an optical microscope examination and 7 genetic characteristics of hybrid strains are different from parent strains by Antagonism experiment. The results of agronomic traits comparison showed that cultivation period, diameter and the yield of hybrid strains GT3 are significantly different from the parents, and the production has exceeded the parent strains more than 3%. GT3 can be used as an excellent hybrid strains reserves and has the potential to develop into a new species. Sexual hybridization breeding would be effective way to select good quality strain.
出处
《生物技术通报》
CAS
CSCD
北大核心
2015年第2期148-152,共5页
Biotechnology Bulletin
基金
重庆市科技攻关项目(JA-12-21-1)
关键词
银耳
单孢分离
有性杂交
优良菌株
Tremella fuciformis
single spore isolation
sexual hybridization
good quality strain
