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组蛋白去甲基化酶KDM5A在人牙髓细胞及成牙本质细胞分化中的表达 被引量：2

Expression of histone demethylase KDM5A in human dental pulp cells during odontogenic differentiation process
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摘要 目的探讨组蛋白赖氨酸去甲基化酶KDM5A在人牙髓细胞（hDPC）中的表达模式及成牙本质分化诱导对其表达量的影响。方法体外培养hDPC,实时荧光定量聚合酶链反应和Western blot检测第1代至第8代（P1-P8代）hDPC中KDM5A mRNA和蛋白的表达量;免疫荧光检测KDM5A在hDPC中的分布;对P3代细胞进行成牙本质分化诱导,于第7天和第14天分别检测KDM5A mRNA和蛋白的表达水平。结果体外传代培养hDPC中可检测到KDM5A的表达,KDM5A mRNA和蛋白量均呈先增加后减少的趋势;hDPC细胞质及细胞核中均表达KDM5A;成牙本质向分化诱导7和14 d,KDM5A mRNA和蛋白量高于未诱导组细胞,诱导14 d表达量高于诱导7 d（P〈0.05）。结论 hDPC表达KDM5A,矿化诱导可提高KDM5A的表达。 Objective To investigate the expression of KDM5 A in human dental pulp cells during in vitro odontogenic differentiation. Methods Human dental pulp cells（hDPCs） were cultured from human normal tooth pulp. KDM5 A mRNA and protein in hDPCs from passage 1 to passage 8（P1-P8） were detected using real-time quantitative PCR and western blot. Celluar KDM5 A localization in hDPCs was determined by immunofluorescence. HDPCs at passage 3 were cultured in the odontoblastic differentiation induction media for 7 d and 14 d, and the mRNA and protein level of KDM5 A were confirmed by real-time quantitative PCR and western blot. Results Real-time quantitative PCR and western blot showed that mRNA and protein expression of KDM5 A increased at passage 1-2 and decreased at passage 7-8 in hDPCs. KDM5 A existed in both the cytoplasm and the nucleus of the hDPCs.KDM5 A mRNA and protein expression gradually increased during the odontogenic differentiation of the hDPCs（P〈0.05）. Conclusion KDM5 A present in hDPCs. Odontogenic differentiation induction enhance the expression level of KDM5 A.
作者 李金铃 廖章松 饶利佳 徐琼
机构地区 中山大学光华口腔医学院·附属口腔医院
出处 《中华口腔医学研究杂志（电子版）》 CAS 2014年第6期18-22,共5页 Chinese Journal of Stomatological Research(Electronic Edition)
关键词 组蛋白去甲基化酶 KDM5A 牙髓细胞 成牙本质分化 Histone demethylases KDM5A Dental pulp cell Odontogenic differentiation
分类号 R346 [医药卫生—基础医学]
  • 相关文献

参考文献34

  • 1Ruthenburg AJ, Allis CD, Wysocka J. Methylation of lysine 4 on histone H3: intricacy of writing and reading a single epigenetic mark [ J ]. Mol Cell, 2007,25 ( 1 ) : 15-30.
  • 2Chfistensen J, Agger K, Cloos PA, et al. RBP2 belongs to a family of demethylases, specific for tri-and dimethylated lysine 4 on histone 3[J]. Cell, 2007,128(6) : 1063-1076.
  • 3Chaturvedi CP, Somasundaram B, Singh K, et al. Maintenance of gene silencing by the coordinate action of the I-I3K9 methyltransferase G9a/KMTIC and the H3K4 demethylase Jaridla./KDM5A[J]. Proc Natl Acad Sci U S A, 2012,109(46) : 18845-18850.
  • 4Gildea JJ, Lopez R, Shearn A. A screen for new trithoraxgroup genes identified little imaginal discs, the Drosophila melanogaster homologue of human retinoblastoma binding protein 2[J]. Genetics, 2000,156(2) :645-663.
  • 5Chicas A, Kapoor A, Wang X, et al. H3K4 demethylation by Jaridla and Jaridlb contributes to retinoblastoma-mediated gene silencing during cellular senescence [J]. Proc Natl Acad Sci U S A, 2012,109(23) : 8971-8976.
  • 6Beshiri ML, Holmes KB, Richter WF, et al. Coordinated repression of cell cycle genes by KDM5A and E2F4 during differentiation[J]. Proc Natl Acad Sci U S A, 2012,109(45): 18499-18504.
  • 7Teng YC, Lee CF, Li YS, et al. Histone dernethylase RBP2 promotes lung tumorigenesis and cancer metastasis [J] Cancer Res, 2013,73(15) :4711-4721.
  • 8Pasini D, Hansen KH, Christensen J, et al. Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2 [J] Genes Dev, 2008,22(10) : 1345-1355.
  • 9Wang S, Fisher K, Pou|in GB. Lineage specific trimethylation of H3 on lysine 4 during C. elegans early embryogenesis [J]. Dev Biol, 2011,355(2) :227-238.
  • 10Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J]. Proc Natl Acad Sci U S A, 2000,97(25):13625-13630.

二级参考文献34

  • 1Ruthenburg AJ, Allis CD, Wysocka J. Methylation of lysine 4 on histone H3: intricacy of writing and reading a single epigenetic mark [ J ]. Mol Cell, 2007,25 ( 1 ) : 15-30.
  • 2Chfistensen J, Agger K, Cloos PA, et al. RBP2 belongs to a family of demethylases, specific for tri-and dimethylated lysine 4 on histone 3[J]. Cell, 2007,128(6) : 1063-1076.
  • 3Chaturvedi CP, Somasundaram B, Singh K, et al. Maintenance of gene silencing by the coordinate action of the I-I3K9 methyltransferase G9a/KMTIC and the H3K4 demethylase Jaridla./KDM5A[J]. Proc Natl Acad Sci U S A, 2012,109(46) : 18845-18850.
  • 4Gildea JJ, Lopez R, Shearn A. A screen for new trithoraxgroup genes identified little imaginal discs, the Drosophila melanogaster homologue of human retinoblastoma binding protein 2[J]. Genetics, 2000,156(2) :645-663.
  • 5Chicas A, Kapoor A, Wang X, et al. H3K4 demethylation by Jaridla and Jaridlb contributes to retinoblastoma-mediated gene silencing during cellular senescence [J]. Proc Natl Acad Sci U S A, 2012,109(23) : 8971-8976.
  • 6Beshiri ML, Holmes KB, Richter WF, et al. Coordinated repression of cell cycle genes by KDM5A and E2F4 during differentiation[J]. Proc Natl Acad Sci U S A, 2012,109(45): 18499-18504.
  • 7Teng YC, Lee CF, Li YS, et al. Histone dernethylase RBP2 promotes lung tumorigenesis and cancer metastasis [J] Cancer Res, 2013,73(15) :4711-4721.
  • 8Pasini D, Hansen KH, Christensen J, et al. Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2 [J] Genes Dev, 2008,22(10) : 1345-1355.
  • 9Wang S, Fisher K, Pou|in GB. Lineage specific trimethylation of H3 on lysine 4 during C. elegans early embryogenesis [J]. Dev Biol, 2011,355(2) :227-238.
  • 10Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J]. Proc Natl Acad Sci U S A, 2000,97(25):13625-13630.

共引文献1

同被引文献34

  • 1Ruthenburg AJ, Allis CD, Wysocka J. Methylation of lysine 4 on histone H3: intricacy of writing and reading a single epigenetic mark [ J ]. Mol Cell, 2007,25 ( 1 ) : 15-30.
  • 2Chfistensen J, Agger K, Cloos PA, et al. RBP2 belongs to a family of demethylases, specific for tri-and dimethylated lysine 4 on histone 3[J]. Cell, 2007,128(6) : 1063-1076.
  • 3Chaturvedi CP, Somasundaram B, Singh K, et al. Maintenance of gene silencing by the coordinate action of the I-I3K9 methyltransferase G9a/KMTIC and the H3K4 demethylase Jaridla./KDM5A[J]. Proc Natl Acad Sci U S A, 2012,109(46) : 18845-18850.
  • 4Gildea JJ, Lopez R, Shearn A. A screen for new trithoraxgroup genes identified little imaginal discs, the Drosophila melanogaster homologue of human retinoblastoma binding protein 2[J]. Genetics, 2000,156(2) :645-663.
  • 5Chicas A, Kapoor A, Wang X, et al. H3K4 demethylation by Jaridla and Jaridlb contributes to retinoblastoma-mediated gene silencing during cellular senescence [J]. Proc Natl Acad Sci U S A, 2012,109(23) : 8971-8976.
  • 6Beshiri ML, Holmes KB, Richter WF, et al. Coordinated repression of cell cycle genes by KDM5A and E2F4 during differentiation[J]. Proc Natl Acad Sci U S A, 2012,109(45): 18499-18504.
  • 7Teng YC, Lee CF, Li YS, et al. Histone dernethylase RBP2 promotes lung tumorigenesis and cancer metastasis [J] Cancer Res, 2013,73(15) :4711-4721.
  • 8Pasini D, Hansen KH, Christensen J, et al. Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2 [J] Genes Dev, 2008,22(10) : 1345-1355.
  • 9Wang S, Fisher K, Pou|in GB. Lineage specific trimethylation of H3 on lysine 4 during C. elegans early embryogenesis [J]. Dev Biol, 2011,355(2) :227-238.
  • 10Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J]. Proc Natl Acad Sci U S A, 2000,97(25):13625-13630.

引证文献2

二级引证文献3

中华口腔医学研究杂志（电子版）
2014年 第6期

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