摘要
本实验研究了阳春砂多糖(AVP)及其纯化组分的抗氧化活性,首先采用DEAE-纤维素-52离子交换柱分级洗脱阳春砂粗多糖,再采用Sephadex G-100葡聚糖凝胶色谱柱进一步纯化得到纯化多糖;分别采用超氧阴离子自由基体系、羟基自由基体系、1,1-二苯基苦基苯肼自由基体系,对阳春砂多糖的抗氧化活性进行研究,并以维生素C为阳性对照物,实验结果表明阳春砂粗多糖及纯化组分对超氧阴离子自由基、羟基自由基、1,1-二苯基苦基苯肼自由基均有较强的清除能力,AVP-3体外抗氧化活性最强。
In the present study,the antioxidant activities of Amomum villosum polysaccharide（ AVP） and its purified product were investigated. The crude AVP was purified by DEAE-cellulose-52 ion exchange column chromatography and further purified through a Sephadex G-100 column. The antioxidant activities of AVP and its purified fractions were then tested using superoxide anion radical system,hydroxyl radical system,1,1-diphenyl picrylhydrazyl radical system with vitamin C as a positive control. The results showed that AVP and its purified fractions showed strong scavenging ability on superoxide anion free radicals,hydroxyl radical and 1,1-diphenyl picrylhydrazyl radical,in which AVP-3 showed the strongest antioxidant activity.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第12期2037-2040,共4页
Natural Product Research and Development
基金
国家科技部"十二五"国家科技支撑计划(2011BAI01B02)
关键词
阳春砂多糖
抗氧化
自由基
Amomum villosum polysaccharide
antioxidant activity
free radical
