摘要
目的研究p62基因在黑素瘤A375细胞生长增殖及细胞周期调控等方面的作用。方法取对数生长期的A375细胞,用干扰p62基因表达的siRNA慢病毒载体转染细胞;MTT法检测转染及正常A375细胞的平均OD值;流式细胞术检测两组的细胞周期分布。结果转染组的平均OD值较正常组小(P<0.05);转染组的G0/G1期比例(0.73±0.01)较正常组(0.64±0.02)高(P=0.002);转染组的凋亡率(0.10±0.02)较正常组(0.03±0.01)高(P=0.004)。结论干扰p62表达后,细胞周期阻滞于G0/G1期,p62在维持细胞周期和肿瘤细胞凋亡中起着重要的作用。
Objective To transfect A375 cells with siRNA of p62 gene, and to explore the role of p62 in A375 cells proliferation, cell cycle and so on. Methods A375 cells were cultured at logarithmic growth phase, and then transfected with siRNA of p62 with lentiviral vectors. MTT assays were performed to detect the OD value in transfected and normal cells;Flow cytometry was performed to detect cell cycle distribution and apoptosis in two groups. Results siRNA was successfully transfected to A375 cells;The OD value of interfering group was lower than that of normal group（P 〈 O. 05） ;The G0/G1 phase ratio of interfering group was higher than that of normal group （ P = 0.002） ; The apoptosis rate of interfering group was higher than that of normal group （P = 0. 004）. Conclusion p62 plays an important role in proliferation of A375 cells. When it was interfered,the cell cycle arrested at G0/G1 phase and the cell apoptosis increased,p62 plays an important role in maintenance of cell cycle and inhibition of cells apoptosis.
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2015年第1期9-11,60,共4页
The Chinese Journal of Dermatovenereology
基金
广东省科技计划项目资助(2010B031600050)
广东省自然科学基金(10451040701005294)
国家自然科学基金(81102599)
