摘要
[目的]希金斯炭疽菌可引起菜心、萝卜等十字花科植物的炭疽病,给经济产生巨大损失。目前,炭疽病菌对苯并咪唑类杀菌剂所产生的抗性问题引起了学者广泛关注,有待于开发新的作用靶标的化学药剂。腺苷酸环化酶(AC)在G蛋白信号传导途径上发挥着重要作用。[方法]基于酿酒酵母中Srv2序列,通过Blastp比对及关键词搜索,并利用SMART进行保守结构域分析,同时,通过对该菌中AC相关蛋白序列进行理化性质、信号肽、跨膜结构域等分析。[结果]明确该菌含有2个与Srv2同源的AC相关蛋白Cg Cap1、Cg Cap2,就理化性质、二级结构等特征方面而言,Cg Cap1、Cg Cap2与Srv2有着较大的差异。此外,通过对Srv2与Cg Cap1、Cg Cap2及其同源序列进行比对分析,发现Cg Cap1、Cg Cap2序列分别与其同源序列及Srv2的C端、N端具有较大的相似性,推测上述序列分别为希金斯炭疽菌AC的C端和N端。[结论]该研究为深入开展该菌AC的研究打下坚实的理论基础。
[Objective] Colletotrichum higginsianum can infect flowering Chinese cabbage, carrot and other cruciferous vegetables,which can cause huge economic losses to the production. Traditionally, to control the pathogen, the benzimidazole fungicides were used, and now this pathogen produce a more serious resistance to ones. So the fungicide with new mechanisms or role of pharmaceutical targets for the pathogen is urgent needed to develop. Adenylate cyclase （AC） as an enzyme plays important role in G protein signaling. [ Methods ] Based on the typical AC sequence have been reported in Saccharomyces cerevisiaeto search AC associated protein sequence from the protein databases of Colletotrichum spp. with the Blastp as well as the use of words, and to search the conserved domain in the SMART online. Meanwhile, bioinformatics analysis have made including the physicochemical propertiessignal peptidetrans - membrane domain structure and the secondary structure. [ Results] There are two AC associated proteins homologous with Srv2 in C. higginsianum, named CgCapl and CgCap2. It is different between CgCapl, CgCap2 and Srv2 on the physicochemical properties, secondary structure. In addition,analysis of genetic relationships through Srv2 and CgCapl, CgCap2 and its homologous sequences, the results showed that, CgCapl, CgCap2 sequences were similar with C- terminal and N- terminal of Srv2 ,respectively ,which suggests that CgCapl, CgCap2 sequences are the C -terminal and N - terminal of the AC in C. higginsianum, respectively. [ Conclusion ] So these studies provide strong theoretical foundation to further study on AC.
出处
《生物技术》
CAS
CSCD
北大核心
2014年第6期56-60,共5页
Biotechnology
基金
云南省优势特色重点学科生物学一级学科建设项目(编号:50097505)
云南省高校林下生物资源保护及利用科技创新团队(编号:2014015)
云南省教育厅科学研究基金项目("核桃炭疽病菌Gα-GTP的效应分子在生长发育与致病过程中的功能研究"
编号:2014Y330)资助
关键词
希金斯炭疽菌
腺苷酸环化酶相关蛋白
理化性质
亚细胞定位
二级结构
Colletotrichum higginsanum, AC associated protein, physicochemical properties, subcellular localization, secondary structure
