摘要
目的探讨不同强度运动对全脑缺血再灌注大鼠学习能力及海马区氧自由基代谢的影响。方法 60只雄性Sprague-Dawley大鼠随机分成假手术组(n=15)、全脑缺血再灌注组(n=15)、有氧运动预处理组(n=15)、力竭运动预处理组(n=15)。分别在术后1 d、3 d、7 d,HE染色观察海马区神经细胞形态变化,穿梭箱评测大鼠学习能力,羟胺法测定大鼠脑组织海马区超氧化物歧化酶的活性,硫代巴比妥酸法测定丙二醛水平。结果与假手术组比较,其他各组存活的神经元数目、主动回避反应率、超氧化物歧化酶活性显著降低(P<0.001),被动回避潜伏期、丙二醛水平显著升高(P<0.001)。其中,全脑缺血再灌注组存活的神经元数目、主动回避反应率及超氧化物歧化酶的活性显著低于有氧运动预处理组(P<0.001),显著高于力竭运动预处理组(P<0.001),被动回避潜伏期及丙二醛水平显著高于有氧运动预处理组(P<0.001),低于力竭运动预处理组(P<0.001)。结论规律的有氧运动有利于保护脑缺血大鼠的学习能力,而力竭运动则产生负面影响,可能与运动对氧自由基代谢的调节有关。
Objective To explore the effetc of different intensity of exercise on learning ablility and oxygen free radical metabolism in rats after cerebral ischemia-reperfusion （I/R）. Methods 60 male Sprague-Dawley rats were randomly divided into sham group, I/R group, aerobic exercise preconditioning group and exhaustive exercise preconditioning group. The morphological changes of neural cells in hippo-campus were observed with HE staining, the learning ablility was assessed with shuttle box, the activity of superoxide dismutase and malo-ndialdehyde level in hippocampus were measured with hydroxylamine method and TBA method respectively 1, 3, 7 days after injury. Re-sults The number of survival neurons, active avoidance reaction and activity of superoxide dismutase decreased, and the latency of passive avoidance and malondialdehyde levels increased in all the other groups compare with the sham group （P〈0.001）. Further more, the number of surviving neurons, active avoidance reaction rate and the activity of superoxide dismutase were less in the I/R group than in the aerobic exercise preconditioning group （P〈0.001）, and more than in the exhaustive exercise preconditioning group （P〈0.001）, while the latency of passive avoidance and the level of malondialdehyde was more than in the aerobic exercise preconditioning group （P〈0.001）, and less than in the exhaustive exercise preconditioning group （P〈0.001）. Conclusion Regular aerobic exercise is beneficial to protect the learning ability from cerebral I/R in rats, but exhaustive exercise may be negative, which may associated with the metabolism of oxygen free radical in hip-pocampus impacted by exercise.
出处
《中国康复理论与实践》
CSCD
北大核心
2015年第1期26-30,共5页
Chinese Journal of Rehabilitation Theory and Practice
基金
河北省教育厅重点资助项目(No.ZH2012046)
关键词
脑缺血再灌注
学习
有氧运动
力竭运动
氧自由基
大鼠
cerebral ischemia-reperfusion
learning
aerobic exercise
exhaustive exercise
oxygen free radical
rats
