摘要
目的：建立同时测定复方北豆根氨酚那敏片中绿原酸、咖啡酸和蒙花苷含量的方法。方法：采用高效液相色谱法。色谱柱为Agilent ZORBAX SB-C18,流动相为乙腈-0.5%磷酸溶液（梯度洗脱）,检测波长为326 nm,柱温为25℃,流速为1.0 ml/min,进样量为10μl。结果：绿原酸、咖啡酸和蒙花苷的质量浓度分别在3.17~79.20、0.10~2.57、0.60~15.05μg/ml范围内与各自峰面积积分值呈良好线性关系（r=0.999 9、0.999 9、0.999 7）;精密度、稳定性、重复性试验的RSD≤1.59%;绿原酸、咖啡酸和蒙花苷的平均加样回收率分别为98.89%、97.78%、98.70%,RSD分别为0.7%、1.3%、0.3%（n=6）。结论：该方法简便、快速,结果准确、可靠,可用于复方北豆根氨酚那敏片的质量控制。
OBJECTIVE： To establish a method for the content determination of chlorogenic acid, caffeic acid and linarin in Compound paracetamel tablets. METHODS： HPLC method was adopted. The determination was performed on Agilent ZORBAX SB-CI8 column with mobile phase consisted of acetonitrile-0.5% phosphoric acid （gradient elution） at flow rate of 1.0 ml/min. The detection wavelength was set at 326 nm, and column temperature was 25 ℃. The sample size was 10 gl. RESULTS： The linear range were 3.17-79.20 μg/ml of chlorogenic acid （r=0.999 9）, 0.10-2.57 μg/ml for caffeic acid （r=0.999 9） and 0.60-15.05 pg/ml for linarin （r=0.999 9）. RSDs of precision, stability and repeatabillity tests were ≤1.59%. The average recoveries were 98.89% （RSD=0.7%, n=6）, 97.78% （RSD= 1.3%, n=6） and 98.70% （RSD=0.3%, n=6）. CONCLUSIONS ： The method is simple, rapid, accurate and reliable, and can provide reference for quality control of Compound paracetamel tablets.
出处
《中国药房》
CAS
北大核心
2015年第3期416-418,共3页
China Pharmacy
