摘要
目的探讨双酚A(BPA)对雄性小鼠睾丸和附睾中电压依赖性T型钙离子通道(Cav3)的影响及雌激素受体(ERs)的作用特点。方法将野生型(WT)小鼠、雌激素受体基因敲除(αERKO和βERKO)小鼠随机分为对照组和BPA组[100μg/(kg·d)],连续经口灌胃染毒30 d后,荧光定量PCR法检测睾丸和附睾中Cav3.1、Cav3.2和Cav3.3 mRNA表达的变化。结果 BPA可使小鼠睾丸中Cav3.1、Cav3.2和Cav3.3 mRNA表达水平升高,附睾中Cav3.1和Cav3.2 mRNA表达水平升高,但Cav3.3 mRNA表达水平降低。应用ERKO小鼠发现BPA通过ERα和ERβ影响附睾Cav3.1和Cav3.2 mRNA表达,对睾丸Cav3.1、Cav3.2和Cav3.3以及附睾Cav3.3 mRNA的调控则依赖于ERβ。结论 BPA可以影响雄性小鼠睾丸和附睾中电压依赖性T型钙通道3种亚型的基因表达,ERα和ERβ在此过程中发挥了重要作用。
Objective To explore the possible effects of bisphenol A （BPA） on voltage-dependent T-type calcium channels in mouse testis and epididymis, the role of the two ERs was also investigated. Methods Adult male wild type （WT） , ERs knock out mice （cxERKO and [3ERKO） were randomly divided into control and BPA [ 100 p^g/（ kg. d） for 30 days via intragastric administration ] treatment groups. Quantitative real time PCR was used to examine Cav3.1, Cav3.2 and Car3.3 genes of the testis and epididymis. Results In the testis, the mRNA expression of Cav3. 1, Cav3.2 and Cav3.3 were increased in BPA-treated animals. In the epididymis, the mRNA expression of Cav3.1 and Cav3.2 were increased, however, Cav3.3 were decreased in BPA-treated animals. BPA up-regulation of Cav3.1 and Cav3.2 mRNA expression of the epididymis were dependent on both ERa and ER[3, whereas BPA regulation of Cav 3.1 - Cav3.3 of the testis and Cav3.3 of the epididymis appeared to be dependent on ER[3 only. Conclusion It suggested that the ERs signalling was necessary for the regulation of the Cav3 channels mRNA expression by BPA, which could affect the male reproductive function.
出处
《卫生研究》
CAS
CSCD
北大核心
2015年第1期23-27,共5页
Journal of Hygiene Research
基金
国家自然科学基金(No.81072329)
江苏大学高级人才基金资助项目(No.12JDG083)
江苏省博士后科研资助计划项目(No.1301045B)
