摘要
目的：采用高效液相色谱法在不同条件下测定清胃止痛微丸中盐酸小檗碱和芍药苷的含量.方法：盐酸小檗碱检测色谱柱为Diamonsil-C18（250 mm ×4.6 mm,5μm）,流动相为乙腈-0.033 mol· L^-1磷酸二氢钾（30∶70）,检测波长为345 nm;芍药苷检测色谱柱为Shim-pack C18（150 mm ×4.6 mm,5μm）,流动相为乙腈-0.3％磷酸水（12.7∶87.3）,检测波长为230 nm.结果：盐酸小檗碱进样量在0.016 ～0.080 μg与峰面积呈良好线性关系（r =0.999 8）,平均回收率为98.68％,RSD=0.74％;芍药苷在进样量0.268 ～1.340 μg与峰面积呈良好线性关系（r =0.999 9）,平均回收率为99.03％,RSD=0.79％.结论：该方法简便、准确、灵敏,重复性好,可用于清胃止痛微丸的质量控制.
Objective： To determine the content of berberine hydrochloride and paeoniflorin in Qingweizhitong pellet by high performance liquid chromatography in different conditions. Methods： The determination for berberine hydrochloride was conducted by Diamonsil-C18 column （250 mm ×4.6 mm,5μm）. The mobile phase was acetonitrile -0. 033 mol. L^-1 potassium phosphate monobasic （30：70 for volume）. The detection wavelength was 345 nm. The determination for paeoniflorin was conducted by Shimpack C18 （ 150 mm mm ×4.6 mm,5μm ） .The mobile phase was acetonitrile - 0. 3% phosphoric acid （ 12.7：87.3 for volume）. The detection wavelength was 230 nm. Results： Good linear relationship between area and amount was noted in the range of 0. 016-0. 080 μg for berberine hydrochloride with a correlation coefficient of 0. 999 8. The average recovery of berberine hydrochloride was 98.68%, and the RSD was 0. 74%. Good linear relationship between area and amount was noted for 0. 268-1. 340 μg for paeoniflorin with a correlation coefficient of 0. 999 9. The average recovery of paeoniflorin was 99.03%, and the RSD was 0. 79%. Conclusion： The established method is simple, accurate, sensitive and good repeatability, which can be applied for the quality control of Qingweizhitong pellet.
出处
《中国现代中药》
CAS
2014年第12期1027-1030,共4页
Modern Chinese Medicine
