摘要
以不同中草药配制的药物饵料投喂大黄鱼,比较大黄鱼的白细胞吞噬活性、血清溶菌酶、超氧化物歧化酶、过氧化氢酶等非特异性免疫指标及对致病性假单胞菌人工感染抵抗力的差异。试验结果表明,投喂不同中草药饲料28d内,血清溶菌酶、超氧化物歧化酶、过氧化氢酶均随饲料投喂时间的延长而升高;投喂28d后,各试验组血清溶菌酶活性、超氧化物歧化酶活性、过氧化氢酶活性均显著高于对照组(P<0.05),其中党参组大黄鱼超氧化物歧化酶和过氧化氢酶活性显著高于其他组(P<0.05);在假单胞菌人工感染中,黄芩组、黄芪组和党参组表现出一定水平的保护力,其他中药组没有明显保护效果。本研究提示,饲料中添加黄芪、黄芩和党参能有效提高大黄鱼非特异性免疫功能,但不足以保护免于特异性的感染。
The authors compared several nonspecific immunity related parameters ,phagocytosis activity of lymphocytes ,and the activities of serum lysozyme ,superoxide dismutase （SOD） ,and catalase （CAT） of the large yellow croaker （Pseudosciaena crocea） fed the diets containing various Chinese herbal medicines , and assayed the resistance against artificial challenge of pathogenic Pseudomonas plecoglossicida .The re‐sults showed that the activities of serum lysozyme ,SOD and CAT increased with the extension of feeding time within 28 days ;at the 28th day ,the detected enzyme activity in all the tested groups were significant‐ly higher than in control group （P〈0 .05）;and the activities of SOD and CAT in Codonopsis pilosula ad‐ministrated group was significantly higher than in other groups （P〈0 .05） .During the artificial challenge againstpathogenic P.plecoglossicida,in Scutellariaradix ,Astragalusmembranaceus and C.pilosula groups a certain protection shown ,but no obvious protection was recorded in other groups .The findings suggests that w as Chinese herbal medicine additives could effectively enhance the nonspecific immunity of large yellow croaker ,but not enough to protect the fish from specific infection .
出处
《水产科学》
CAS
北大核心
2014年第11期718-722,共5页
Fisheries Science
关键词
大黄鱼
中草药
非特异性免疫
Pseudosciaena crocea
Chinese herbal medicine
nonspecific immunity
