摘要
目的观察力竭运动对大鼠心肌线粒体生物发生关键调控因子过氧化物酶增殖物激活受体γ辅助激活因子-1α(peroxisome proliferator-activated receptorγcoactivator 1α,PGC-1α)、核呼吸因子-1(nuclear respiratory factor-1,NRF-1)和核呼吸因子-2(nuclear respiratory factor-2,NRF-2)蛋白表达的影响及红景天苷(Salidroside,SAL)对这些因子的干预作用。方法将104只雄性SD大鼠随机分为对照(C)组、力竭(EE)组(即刻、6 h、12 h、24 h)、低剂量SAL力竭(LS)组(即刻、6 h、12 h、24 h)、高剂量SAL力竭(HS)组(即刻、6 h、12 h、24 h),每组8只。C组及EE组予生理盐水(10 ml/kg)灌胃2周,LS和HS组分别给予SAL 100、300 mg/kg灌胃2周,EE、LS和HS组灌胃结束后建立力竭游泳模型。比较EE、LS、HS组力竭游泳时间,测定对照组及力竭运动后各组不同时相心肌PGC-1α、NRF-1、NRF-2蛋白的表达。结果 1LS、HS组大鼠游泳时间均较EE组明显延长(P<0.05),HS组较LS组延长得更为显著(P<0.05)。2PGC-1α蛋白表达量均为即刻最低,6 h最高,差异有统计学意义(P<0.05);NRF-2蛋白的表达量均为即刻最低,12 h最高,差异有统计学意义(P<0.05);NRF-1蛋白的表达量无明显的时间趋势。与C组比较,EE组各时相心肌PGC-1α蛋白的表达量均显著降低(P<0.05),即刻、6 h、24 h组心肌NRF-1和NRF-2蛋白的表达量显著降低(P<0.05)。与相同时相的EE组比较,LS组即刻、6 h、24 h PGC-1α蛋白表达量,6、12、24 h心肌NRF-1蛋白表达量,即刻、6 h、12 h NRF-2蛋白表达量均显著增高(P<0.05);HS组PGC-1α及NRF-2蛋白表达量均显著增高(P<0.05),即刻、12 h组心肌NRF-1蛋白的表达量均显著降低(P<0.05)。与相同时相的LS组比较,HS组即刻、24 h PGC-1α蛋白表达量显著降低,6、12 h显著增高(P<0.05);HS组各时相心肌NRF-1蛋白表达量均显著降低(P<0.05);HS组6、12、24 h心肌NRF-2蛋白表达量均显著增高(P<0.05)。结论 SAL能通过刺激力竭大鼠心肌PGC-1α、NRF-2蛋白表达的上调诱导线粒体生物发生,起到保护心肌的作用。
Objective To observe the influence of exhaustive swimming exercise on the expressions of key regulatory factors of mitochondrial biogenesis such as peroxisome proliferator-activated receptor γ coactivator 1 α（ PGC-1α）,nuclear respiratory factor-1（ NRF-1） and nuclear respiratory factor-2（ NRF-2） protein and intervention effect of Salidroside（ SAL） on the factors. Methods A total of 104 health male Sprague Dawley rats were randomly divided into 4groups： sedentary control group（ C group,n = 8）; exhausting swimming exercise group（ EE group）; low-dose SAL and exhausting exercise group（ LS group） and high-dose SAL and exhausting exercise group（ HS group）. The EE,LS and HS groups were divided into 4 subgroups respectively according to 0 h,6 h,12 h and 24 h after exhausting exercise（ n= 8 in each subgroup）. C and EE groups were lavaged with physiological saline（ 10 ml / kg） for 2 weeks,while LS and HS groups were lavaged with low-dose SAL（ 100 mg / kg） or high-dose SAL（ 300 mg / kg） respectively for 2 weeks,and then models of exhausting swimming exercise were established in EE,LS and HS groups. Exhausting swimming times were compared that in EE,LS and HS groups,and expression levels of PGC-1α,NRF-1 and NRF-2 protein were detected in different phases in all the groups. Results Exhausting swimming times of LS and HS groups were significantly longer than that of EE group（ P〈0. 05）,while the extended itme of HS group was more significant than that of LS group（ P〈0. 05）. The expression of PGC-1α protein in the 0 h subgroups were the lowest,while the highest expression level was found in 6 h subgroups,and there were significant differences（ P〈0. 05）; expression of NRF-2 protein in 0 h subgroups were the lowest,while 12 h subgroups were the highest,and there were significant differences（ P〈0. 05）; the expression of NRF-1 had no significant time tendency. Compared with those in C group,the expressions of myocardial PGC-1α protein in EE subgroups were significantly decreased（ P〈0. 05）,and the expressions of NRF-1 and NRF-2 protein in 0 h,6 h and 24 h subgroups were decreased significantly（ P〈0. 05）. Compared with those at the same time of EE subgroup,in LS group,the expressions of PGC-1α protein in 0 h,6 h and 24 h subgroups,NRF-1 protein in 6 h,12 h and 24 h subgroups,and NRF-2 protein in 0 h,6 h and 12 h subgroups were significantly increased（ P〈0. 05）; the expressions of PGC-1 α and NRF-2 protein in HS subgroups were significantly increased（ P〈0. 05）,and the expressions of NRF-1 protein in 0 h and 12 h subgroups were significantly decreased（ P〈0. 05）. Compared with those at the same time of LS subgroups,in HS group,the expressions of PGC-1α protein in 0 h and 24 h subgroups were significantly decreased（ P〈0. 05）,while the expressions in 6 h and 12 h subgroups were significantly increased（ P〈0. 05）; the expressions of NRF-1 protein in HS subgroups were all significantly decreased（ P〈0. 05）; in HS group the expressions of NRF-2 protein in 6 h,12 h and 24 h subgroups were significantly increased（ P〈0. 05）. Conclusion Salidroside may have protective effect of myocardial damage after exhaustive exercise by promoting mitochondrial biogenesis induced by stimulating the increase of PGC-1α and NRF-2 protein expressions.
出处
《解放军医药杂志》
CAS
2014年第11期6-10,共5页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
国家自然科学基金资助项目(81173585)
