摘要
目的:观察板桥党参水煎液对溃疡性结肠炎模型大鼠肠黏膜损伤的修复作用。方法:将60只SD大鼠随机分为空白对照组、模型对照组、柳氮磺胺吡啶(SASP)阳性对照组以及板党水煎液高、中、低3个剂量组(600、300、150 mg/kg),每组10只。采用2,4-二硝基氯苯(DNCB)复合乙酸法复制溃疡性结肠炎大鼠模型。造模后给药2周,麻醉处死动物,观察大鼠结肠形态变化并测定大鼠结肠重量、肠重指数及肠组织中超氧化物歧化酶(SOD)和丙二醛(MDA)的含量。结果:与模型对照组比较,板党水煎液组和SASP阳性对照组大鼠结肠组织溃疡面积明显缩小,水肿缓解;结肠重量以及肠重指数均明显下降(P<0.05);肠组织SOD含量明显增加(P<0.05),MDA明显减少(P<0.05)。结论:板党水提物能显著缓解2,4-二硝基氯苯复合乙酸所致慢性溃疡性结肠炎大鼠结肠黏膜损伤程度,其机制可能与提高机体抗氧化能力有关。
Objective： To observe the repair of Ban Qiao Dang Shen [Codonopsis pilosula（Franch.） Nannf.] decoction on the damage of intestinal mucosa of rat model with ulcerative colitis（UC）. Methods： Sixty SD rats were randomized into blank control group, model control group, positive control group of salazosulfapyridine（SASP）,high, moderate and low dose groups of Ban Qiao Dang Shen（600, 300 and 150mg/kg）. Rat model suffering from UC was prepared by DNCB combined with acetic acid method, they were sacrificed after the anesthesia and medicated for two weeks after establishing the models, the contents of SOD and MDA in the intestinal tissue, intestinal weight index and the weight of colons were detected; morphological changes of the colon were observed. Results： Ulcer area of the colon was decreased significantly, the edema was alleviated; colon weight and intestinal weight index were lowered obviously（P〈0.05）; the contents of SOD in intestinal tissue were increased notably but the contents of MDA were reduced when Ban Qiao Dang Shen decoction groups and SASP positive control group were compared with model control group. Conclusion： The decoction of Ban Qiao Dang Shen could remarkably relieve the symptoms of the models with chronic UC which is induced by DNCB combined with acetic acid method, and its mechanism might be related to improve oxidation resistance of the body.
出处
《西部中医药》
2014年第11期11-13,共3页
Western Journal of Traditional Chinese Medicine
