摘要
目的研究白细胞介素1A基因（IL1A）3′-UTR区＂TTCA＂插入（I）/缺失（D）多态性位点与中国汉族男性前列腺癌之间的关系。方法采用聚合酶链反应-聚丙烯酰胺凝胶电泳（PCR-PAGE）方法,在131例前列腺癌患者和229例正常对照者样本中对IL1A基因3′-UTR区＂TTCA＂插入/缺失多态性位点（rs3783553）进行基因分型,分析比较两组样本间等位基因频率及基因型频率分布差异,探讨该位点与前列腺癌的相关性。结果基因分型结果显示,两组样本中rs3783553位点基因型频率分布均符合Hardy-Weinberg平衡;统计结果显示,与D/D基因型相比,基因型为D/I或I/I的个体罹患前列腺癌的风险降低（P〈0.001,OR=0.48,95%CI：0.31～0.74）;相对于等位基因D,等位基因I罹患前列腺的风险降低（P=0.001,OR=0.56,95%CI：0.40～0.79）。结论 IL1A基因3′-UTR区＂TTCA＂四碱基插入/缺失多态性可能与中国汉族男性前列腺癌的发病相关。
Objective To identify the association between the insertion/deletion polymorphism of interleukin-1Agene（IL1A）and the susceptibility of prostate cancer（PCA）.Methods We performed a case-control study enrolling 131 PCA patients and 229 healthy control subjects in a Chinese Han population.The TTCA insertion/deletion polymorphism（rs3783553）in 3′-UTR of IL1 Agene was genotyped by PCR-RFLP method.Results The genotype distribution of rs3783553 in both groups met the requirements of Hardy-Weinberg equilibrium.Significantly reduced PCA risk was associated with D/I and I/I genotype compared to D/D genotype（P〈0.001,OR=0.48,95%CI：0.31-0.74）,and allele I is associated with the reduced PCA risk（P=0.001,OR=0.56,95%CI：0.40-0.79）.Conclusion TTCA insertion allele of rs3783553 contributes to the reduction of the susceptibility to prostate cancer.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2014年第6期956-959,共4页
Journal of Sichuan University(Medical Sciences)
