期刊文献+

鸡卵清提取液中小于3kDa的成分有促细胞增殖的作用 被引量:4

Components of Chicken Ovalbumin Extract Less Than 3 kDa in Size Promote Cell Proliferation
原文传递
导出
摘要 在前期研究中发现,鸡卵清提取液有促细胞存活与增殖的作用。在该研究中,作者进一步分离鸡卵清提取液的各成分,找到促细胞增殖的主要成分。用超滤管将鸡卵清提取液分为大于3 kDa和小于3 kDa的成分,用于细胞增殖活性测定,发现鸡卵清提取液中小于3 kDa的成分有促293T细胞增殖的作用。可将鸡卵清提取液分离小于3 kDa的成分作为促293T细胞增殖的有效成分。 We found that chicken egg extract could promote cell survival and proliferation. In this study, we further separated the components of chicken egg extract to find the main ingredient promoting cell proliferation. The chicken ovalbumin extract was separated into more than 3 kDa and less than 3 kDa components by ultrafiltration, and then their effect on cell proliferation activity were determined. Less than 3 kDa ingredients in chicken ovalbumin extract can promote 293 T cell proliferation. We can separate less than 3 kDa fraction in the chicken ovalbumin as an active ingredient to promote 293 T cell proliferation.
出处 《中国细胞生物学学报》 CAS CSCD 北大核心 2014年第10期1350-1354,共5页 Chinese Journal of Cell Biology
基金 国家自然科学基金(批准号:31172170) 国家重点基础研究发展计划(973计划)(批准号:2012CB518106) 国家支撑计划项目(批准号:2014BAI01B01) 云南省高新技术产业发展专项(批准号:201204)资助的课题~~
关键词 鸡卵清提取液 细胞增殖 小分子物质 chicken ovalbumin extract cell proliferation small molecules substance
  • 相关文献

参考文献15

  • 1Abeyrathne ED,Lee HY,Ahn DU.Separation of ovotransferrinand ovomucoid from chicken egg white.Poult Sci 2014,93(4):1010-7.
  • 2Fang J,Ma MH,Qiu N,Wu X,Jin YG.Analysis of the lowmolecularweight protein profile of egg-white and its changesduring early chicken embryological development.Z NaturforschC 2012,67(3/4):208-14.
  • 3Kodama D,Nishimiya D,Nishijima K,Okino Y,Inayoshi Y,Kojima Y,et al.Chicken oviduct-specific expression of transgeneby a hybrid ovalbumin enhancer and the Tet expressionsystem.J Biosci Bioeng 2012,113(2):146-53.
  • 4Li L,He L,Tan S,Guo X,Lu H,Qi Z,et al.3-hydroxyphthalicanhydride-modified chicken ovalbumin exhibits potent and broadanti-HIV-1 activity:A potential microbicide for preventing sexualtransmission of HIV-1.Antimicrob Agents Chemother 2010;54(5):1700-11.
  • 5Lingappa VR,Lingappa JR,Blobel G.Chicken ovalbumin containsan internal signal sequence.Nature 1979,281(5727):117-21.
  • 6Mayer S,Roeser M,Lachmann P,Ishii S,Suh JM,Harlander S,et al.Chicken ovalbumin upstream promoter transcription factor II regulates renin gene expression.J Biol Chem 2012,287(29):24483-91.
  • 7McReynolds L,O’Malley BW,Nisbet AD,Fothergill JE,Givol D,Fields S,et al.Sequence of chicken ovalbumin mRNA.Nature1978,273(5665):723-8.
  • 8Mizutani A,Tsunashima H,Nishijima K,Sasamoto T,YamadaY,Kojima Y,et al.Genetic modification of a chicken expressionsystem for the galactosylation of therapeutic proteins produced inegg white.Transgenic Res 2012,21(1):63-75.
  • 9Qiu N,Liu W,Ma M,Zhao L,Li Y.Differences between fertilizedand unfertilized chicken egg white proteins revealed by2-dimensional gel electrophoresis-based proteomic analysis.Poult Sci 2013,92(3):782-6.
  • 10Shin SW,Kwon HC,Rho MS,Choi HJ,Kwak JY,Park JI.Clinical significance of chicken ovalbumin upstream promotertranscriptionfactor II expression in human colorectal cancer.Oncol Rep 2009,21(1):101-6.

同被引文献24

  • 1Miyamoto K, Tsukiyama T, Yang Y, et al. CeU-free extracts from mammalian oocytes partially induce nuclear reprogramming in somatic cells [ J ]. Biol Reprod ,2009,80 ( 5 ) :935-943.
  • 2Hansis C, Barreto G, Maltry N, et al. Nuclear reprogramming of human somatic cells by xenopus egg extract requires BRG1 [ J ]. Curt Bio1,2004,14 ( 16 ) : 1475-1480.
  • 3Miyamoto K, Furusawa T, Ohnuki M, et al. Reprogramming events of mammalian somatic ceils induced by Xenopus laevis egg extracts [ J]. Mol Reprod Dev,2007,74( 10):1268-1277.
  • 4LiY(李勇),CaiMY(蔡木易).Peptide Nutrition(肽营养学).Bering:Peking University Medical Press.2007.
  • 5Mizrahi A. Primatone in mammalian cell culture media. Bio- technol Bioeng, 1977,19 : 1557-1561.
  • 6Heidemann R, Zhang C, Qi H, et al. The use of peptones as medium additives for the production of a recombinant thera- peutic protein in high density perfusion cultures of mammali- an cells. Cytotechnology,2000,32 : 157-167.
  • 7Fran~k F, Eckschlager T, Katinger H. Enhancement of mono- clonal antibody production by lysine-containing peptides. Biotechnol Prog ,2003 ,19 : 169-174.
  • 8Fujita H,Sasaki R, Yoshikawa M. Potentiation of the antihy- pertensive activity of orally administered ovokinin, a vasore- laxing peptide derived from egg white protein, by emulsifica- tion in egg phosphatedylcholine. Biosci Biotechnol Biochem, 1995,59 : 2344 -2345.
  • 9Lv L, Chi YJ. Improvement of functional properties of egg white protein phosphorylated by dry-heating in the presence of pyrophosphate. Eur Food Res Technol, 2012,235: 981- 987.
  • 10Menarpoor-yazdi M, Asoodeh A, Chamani J. A novel antioxi- dant and antimicrobial peptide from hen egg white lysozyme hydrolysates. J Funct Foods ,2012,4:278-286.

引证文献4

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部