摘要
目的利用siRNA技术下调GLI1基因的表达,在体外研究GLI1对MKN28胃癌细胞侵袭转移能力的影响。方法脂质体法将GLI1 siRNA转染MKN28胃癌细胞,采用RT-PCR观察GLI1 siRNA转染前后GLI1在mRNA水平的变化情况。培养MKN28胃癌细胞,将细胞分为转染GLI1 siRNA组、阴性siRNA组和对照组后,分别采用Transwell小室法检测各组细胞的侵袭和转移能力。结果转染GLI1 siRNA后GLI1基因表达在24 h和48 h明显下调。侵袭和转移实验显示:GLI1 siRNA组侵袭和转移细胞数目均低于阴性siRNA组和对照组(P<0.05),而阴性siRNA组和对照组间的差异均无统计学意义。结论转染GLI1 siRNA特异下调GLI1基因表达,在体外抑制胃癌MKN28细胞的侵袭和转移,可能是胃癌靶向治疗的靶点。
Objective To investigate the invasion and metastasis ability of GLI1 on MKN28 cells in vitro with siRNA-mediated inhibition of GLI1. Methods siRNA targeting GLI1 mRNA was transfected into MKN28 cells, and GLI1 expression was determined by RT-PCR. MKN28 cell were cultured in vitro and divided into GLI1 siRNA group, negative siRNA group and control group. Invasion and metastasis of MKN28 cells were assessed by transwell chamber assay. Results GLI1 siRNA significantly inhibited the expression of GLI1 in MKN28 cell line at mRNA levels.The number of invasion and metastasis cells of MKN28 cells in GLI1 siRNA group were significantly lower than those in negative siRNA group and control group（all P〈0.05）. Conclusion Transfection of GLI1 can inhibit GLI1 expression, thus suppress cell invasion and metastasis of MKN28 cells. This may provide a new therapy target for gastric cancer.
出处
《中华临床医师杂志（电子版）》
CAS
2014年第18期86-88,共3页
Chinese Journal of Clinicians(Electronic Edition)
基金
上海市科学技术委员重点基础项目(07jc14041)
上海市重点学科(s30204)
国家自然科学基金(30872476)
关键词
胃肿瘤
肿瘤转移
肿瘤浸润
小干扰
GLll
Stomach neoplasms
Neoplasm metastasis
Neoplasm invasiveness
Smallinterferencing
GLI1
