摘要
以浙江省主栽品种农桑14号、强桑1号、荷叶白为对象,研究了不同时间、不同叶片中1-脱氧野尻霉素(DNJ)含量差异以及光照对桑树DNJ含量的影响。结果发现:3个品种桑叶DNJ含量在不同时期差异显著,同一品种不同叶片间DNJ含量同样差异显著。在春季,3个品种嫩芽中DNJ含量分别高达0.432%,0.467%和0.649%;前10片成熟叶DNJ平均含量在5月1日左右最高,此后随着叶片的老化而逐渐降低至0.2%以下。夏伐后,3个品种成熟叶DNJ平均含量在7月1日左右最高,之后随叶片衰老,DNJ含量均逐渐降低,但随着气温升高、光照增强,DNJ含量再次升高,农桑14于9月1日、强桑1号于9月16日、荷叶白于8月16日再次达到峰值,后随叶片进一步衰老而逐渐降低至0.1%以下。农桑14组培苗中DNJ含量测定发现,光照不仅明显影响桑苗生长形态,日光照5 h和16 h桑苗叶片中DNJ含量分别由(0.389±0.029)%提高至(0.689±0.024)%,新梢中由(0.449±0.026)%提高至(0.859±0.037)%。试验结果表明,桑叶DNJ在嫩芽中含量最高,后随叶片老化而降低,但光照可显著提高桑树中DNJ含量,该结果为药用型桑树的栽培区域以及收获时期的选择提供参考。
Using the Zhejiang Province leading varieties of Nongsang 14,Qiangsang 1 and Heyebai as the subjects,this experiment was conducted to investigate the difference of 1-deoxynojirimycin（ DNJ） concentration in different mulberry leaves at different time,and the effect of light on DNJ concentration in mulberry tree. The results showed that the DNJ content in these three varieties of mulberry leaf was significantly different in different periods,and within the same variety,leaf DNJ content also had significant difference. In spring,DNJ content in these three varieties was as high as 0. 432%,0. 467% and 0. 649%,respectively. The highest average concentration of DNJ in the first10 pieces of mature leaves was on May 1^st,thereafter with leaf aging,it gradually decreased to below 0.2%. After cutting in summer,the average concentration of DNJ in the first 10 pieces of mature leaves in these three varieties was the highest on July 1^st. Followed with the leaf senescence,the content of DNJ was decreased gradually. But with temperature and light intensity increased,the content of DNJ increased again,and reached to the peak on September1 stfor Nongsang 14,September 16 thfor Qiangsang 1 and August 16 thfor Heyebai. Thereafter,with leaf aging,the DNJ concentration gradually decreased to below 0. 1%. The effect of light intensity on the DNJ content of NongSang14 plantlets was studied,and the result showed that with 5 h and 16 h light per day,not only the mulberry growth morphology was significantly affected,but the DNJ content was also evidently increased from（0. 389 ± 0. 029）% to（0.689 ±0.024）% in leaf and enhanced from（0.449 ±0.026）% to（0.859 ±0.037）% in trunk. These results indicated that the concentration of the DNJ was the highest in mulberry bud,and with the leaf senescence,its concentration reduced gradually. But the DNJ content in mulberry leaf could significantly increase under longer light condition. These results provided a consult for selecting the cultivation areas and harvest period of medicinal used mulberry trees.
出处
《浙江农业学报》
CSCD
北大核心
2014年第5期1240-1245,共6页
Acta Agriculturae Zhejiangensis
基金
浙江省蚕桑产业科技创新团队(2011R50028)
浙江省蚕桑农业新品种选育重大科技专项(2012C12910)
国家蚕桑产业技术体系(CARS-22-ZJ0105)
浙江省自然科学基金(LQ12H31002)
