摘要
以中华水韭为试验材料,用200μmol·L-1的氯化镉溶液和2g·L-1的硝酸铅分别对其进行胁迫处理,采用cDNA-AFLP技术分离差异表达基因,通过Blast(The Basic Local Alignment Search Tool)同源性比对分析基因功能,为寻找耐重金属胁迫相关基因和揭示水韭对环境变化响应的分子机理奠定基础。试验结果如下:共有15条差异条带被成功克隆和测序,其中13条差异片段可以在数据库找到与之同源的序列。镉胁迫差异表达TDF9与ABC型(ATP-binding cassette)转运蛋白C亚族基因有84%同源性。铅胁迫差异表达TDF4和TDF7与环形锌指蛋白(RING Zinc Finger Protein)有75%同源性。这些基因的发现为揭示水韭对环境变化敏感的分子机理奠定基础。
In this paper,living fossil plant I.sinensis as test materials,200μmol.L-1CdCl2 solution and 2g.L-1 Ph（NO3）2 were carried out on Isoetes sinensis stress treatment,cDNA-AFLP technique were used for separa- tion of deferential expression genes, homology analysis of the genes by Blast （The Basic Local Alignment Search Tool）to find resistance to heavy metal stress-related genes and reveal the molecular mechanism of response to environmental change.The results are as follows： A total of 15 different bands were successful- ly cloned and sequenced, of which 13 differential fragments with homologous sequences can be found in the database. Cd stress differentially expressed TDF9 with ABC-type（ATP-binding cassette）transporter pro- tein C gene subfamily has 84% homology.Lead Stress differentially expressed TDF4 and TDF7 and ring zinc finger protein（RING Zinc Finger Protein）has 75% homology.Discovery of these genes lay foundation to reveal molecular mechanisms of Isoetes sinensis being sensitive to environmental change.
出处
《安徽农学通报》
2014年第18期22-24,共3页
Anhui Agricultural Science Bulletin
基金
哈尔滨师范大学第七届大学生实践创新基金项目
